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In-vitro rapid propagation seedling growing method for double-petal lily

A technology for fast-growing seedlings and lilies, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of affecting the yield of lilies, low reproduction coefficient, etc., and achieve the effect of increasing the reproduction speed

Active Publication Date: 2022-04-12
山东百悦农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Existing double lilies are generally propagated by methods such as dividing balls, dividing bulbils, and scale cuttings, but the reproduction coefficient is low, thereby affecting the yield of lilies; plant tissue culture Technology is one of the important research techniques and means in life sciences

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for rapid breeding of lily double in vitro, comprising the following steps:

[0029] S1, culture body selection: select the lily stem segment with tender buds, and cut the stem segment from the main trunk;

[0030] S2, stem section treatment: use a cotton ball dipped in alcohol with a concentration of 75% to wipe the incision of the stem section for disinfection and sterilization;

[0031] S3, stem segment culture: cut off the lower part of the stem segment, and put the stem segment into medium Ⅰ;

[0032] S4, induction culture: After 3 new shoots appeared in the stem segment in step 3, the stem segment was evenly divided into 3 segments, and then placed in medium II for induction culture. After the new shoots grew, repeat this step to achieve Continuous induction of proliferation;

[0033] S5, swelling and rooting. In step 4, the new shoots are cultured in medium II for 20 days and then transferred to medium III to accelerate the growth and rooting of shoots...

Embodiment 2

[0042] A method for rapid breeding of lily double in vitro, comprising the following steps:

[0043] S1, culture body selection: select the lily stem segment with tender buds, and cut the stem segment from the main trunk;

[0044] S2, stem section treatment: use a cotton ball dipped in alcohol with a concentration of 75% to wipe the incision of the stem section for disinfection and sterilization;

[0045] S3, stem segment culture: cut off the lower part of the stem segment, and put the stem segment into medium Ⅰ;

[0046] S4, induction culture: After 3 new shoots appeared in the stem segment in step 3, the stem segment was evenly divided into 3 segments, and then placed in medium II for induction culture. After the new shoots grew, repeat this step to achieve Continuous induction of proliferation;

[0047] S5, swelling and rooting. In step 4, the new shoots are cultured in medium II for 20 days and then transferred to medium III to accelerate the growth and rooting of shoots...

Embodiment 3

[0056] A method for rapid breeding of lily double in vitro, comprising the following steps:

[0057] S1, culture body selection: select the lily stem segment with tender buds, and cut the stem segment from the main trunk;

[0058] S2, stem section treatment: use a cotton ball dipped in alcohol with a concentration of 75% to wipe the incision of the stem section for disinfection and sterilization;

[0059] S3, stem segment culture: cut off the lower part of the stem segment, and put the stem segment into medium Ⅰ;

[0060] S4, induction culture: After 3 new buds appeared in the stem segment in step 3, the stem segment was evenly divided into 3 segments, and then placed in medium II for induction culture. After the new buds grew, repeat this step to achieve Continuous induction of proliferation;

[0061] S5, swelling and rooting. In step 4, the new shoots are cultured in medium II for 20 days and then transferred to medium III to accelerate the growth and rooting of shoots.

...

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PUM

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Abstract

The invention discloses a double-petal lily in-vitro rapid propagation seedling growing method which comprises the following steps: S1, selecting a culture body: selecting a lily stem section with a tender shoot, and cutting and taking down the stem section from a trunk; s2, stem section treatment: dipping a cotton ball in alcohol with the concentration of 75% to wipe the cut part of the stem section, and performing disinfection and sterilization treatment; s3, stem section culture: cutting off the lower end part of a stem section, and putting the stem section into a culture medium I; and S4, induction culture: after 1-4 new buds appear on the stem segment in the step 3, uniformly dividing the stem segment into 2-4 segments, then putting the stem segment into a culture medium II for induction culture, and after the new buds grow, repeating the step to realize continuous induction proliferation. According to the method, the stem segment of the double-petal lily is cut to serve as a culture body, the adventitious bud is induced after disinfection, and the tissue culture seedling is obtained through proliferation and rooting of the adventitious bud, so that in-vitro rapid propagation and culture of the seedling are realized, and the propagation speed of the double-petal lily is greatly increased.

Description

technical field [0001] The invention relates to the technical field of lily cultivation, in particular to a method for in vitro rapid propagation of double-petal lily seedlings. Background technique [0002] Lily is a perennial herb of the genus Lilium in the family Liliaceae, with underground bulbs. As a flower with high ornamental value, it is suitable for gardens, flower beds and potted plants. It is deeply loved by people for its meaning of "a hundred years of harmony", and it has become one of the best-selling flowers in the market; as one of the four fresh cut flowers 1. The sales volume of lily bulbs and cut flowers has always been among the top in the world. It has the reputation of "the king of flower bulbs". Double lily is a new horticultural variety of lily with high ornamental and economic value. [0003] Existing double-petal lilies are generally propagated by methods such as dividing balls, dividing bulbils, and scale cuttings, but the reproduction coefficient...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCY02P60/40
Inventor 刘晓华李压凡辛奇乔琳芝
Owner 山东百悦农业科技有限公司
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