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Application of lactobacillus rhamnosus in preparation of food or medicine for preventing and/or treating ulcerative colitis

A technology for ulcerative colitis and Lactobacillus is applied in the field of microorganisms to achieve the effects of improving colonic mucosal inflammatory lesions, improving content, and reducing colon histopathological scores

Pending Publication Date: 2022-04-01
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the treatment of UC with probiotics is still in the development stage

Method used

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  • Application of lactobacillus rhamnosus in preparation of food or medicine for preventing and/or treating ulcerative colitis
  • Application of lactobacillus rhamnosus in preparation of food or medicine for preventing and/or treating ulcerative colitis
  • Application of lactobacillus rhamnosus in preparation of food or medicine for preventing and/or treating ulcerative colitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Preparation of Lactobacillus rhamnosus LRa05 freeze-dried powder:

[0062] Lactobacillus rhamnosus LRa05 was inoculated into the medium according to the inoculum amount accounting for 3% of the total mass of the medium, and cultured at 37°C for 18 hours to obtain a culture solution; the culture solution was centrifuged to obtain a thallus; the thallus was treated with trehalose Lyoprotectant (containing trehalose 100g / L, skimmed milk powder 100g / L) was resuspended (the mass ratio of lyoprotectant to bacteria was 2:1) to obtain a resuspension; the resuspension was vacuum frozen Freeze-drying was carried out to obtain Lactobacillus rhamnosus LRa05 freeze-dried powder, and the number of viable bacteria was detected to be 200 billion CFU / g.

Embodiment 2

[0064] Exploring the effect of Lactobacillus rhamnosus LRa05 on body weight, disease activity index (DAI), colon length and histopathology, myeloperoxidase (MPO) content, oxidative stress marker content, iNOS and COX in mice with ulcerative colitis -2 levels, effects of serum pro-inflammatory cytokines:

[0065] (1) Animal grouping and establishment of ulcerative colitis mouse model:

[0066] The C57BL / 6 mice were randomly divided into 3 groups, 10 in each group, which were normal control group (CTL group), UC model group (UC group) and Lactobacillus rhamnosus LRa05 group (LRa05 group).

[0067] Experimental colitis was induced by oral administration of 3% dextran sodium sulfate DSS (w / v) in drinking water for 7 days. The normal group drank water every day for one week, and the other two groups were given 3% dextran sodium sulfate (w / v) once a day for one week. At the same time, the mice in the Lactobacillus rhamnosus LRa05 group were orally administered the lyophilized powd...

Embodiment 3

[0087] The present embodiment provides a solid drink with the effect of relieving ulcerative colitis, the preparation method is: mixing 0.1 g of Lactobacillus rhamnosus freeze-dried powder obtained in Example 3 with 1.9 g of inulin (inulin) and homogenizing, Made into sachets for brewing. Based on 2.0 grams per bag, this product contains 2 billion live CFU of Lactobacillus rhamnosus LRa05 per bag.

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Abstract

The invention relates to application of lactobacillus rhamnosus to preparation of food or medicine for preventing and / or treating ulcerative colitis. The lactobacillus rhamnosus with the effect of relieving the ulcerative colitis is named as lactobacillus rhamnosus LRa05 strain, the preservation unit is China General Microbiological Culture Collection Center, the preservation time is July 20, 2020, the preservation number is CGMCC No.1. 12734, and the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The lactobacillus rhamnosus LRa05 strain can be used for remarkably relieving the ulcerative colitis and remarkably improving the colonic mucosa inflammation lesion, and the lactobacillus rhamnosus LRa05 strain has a good prospect when being used for preparing the food or the medicine for preventing and / or treating the ulcerative colitis or the colonic mucosa inflammation.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a new medicinal application of Lactobacillus rhamnosus, in particular to an application of Lactobacillus rhamnosus in preparing food or medicine for preventing and / or treating ulcerative colitis . Background technique [0002] Ulcerative colitis (UC) is a chronic inflammatory disease that occurs in the rectum and colon, and belongs to inflammatory bowel disease. It is a chronic intestinal inflammation caused by a variety of reasons, such as genetic factors, lifestyle, and disruption of the colonic microbial ecosystem. UC is clinically manifested as chronic diarrhea, persistent or reproducible mucus, abscess, and blood in the stool and other symptoms of varying degrees. Notably, its protracted disease course generally leads to complications, even colorectal cancer. In addition, its incidence is on the rise and tends to be younger. Although patients can take drugs to contr...

Claims

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Application Information

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IPC IPC(8): A23L33/135A61K9/19A61K35/747A61K47/26A61P1/00A61P1/04C12N1/20C12N1/04C12R1/225
Inventor 方曙光盖忠辉朱建国陈珂可
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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