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Infusion cell freezing medium and application thereof

A cryopreservation solution and cell technology, applied in the field of cell and cell engineering, can solve the problems of allergy and unstable cell cryopreservation solution, and achieve the effects of high safety, good viability and killing ability, and easy operation.

Active Publication Date: 2022-04-01
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] As a substance whose composition is not yet clear, serum has certain batch-to-batch differences, which leads to unstable effect of cell cryopreservation solution, and clinical use may cause lead to allergies

Method used

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  • Infusion cell freezing medium and application thereof
  • Infusion cell freezing medium and application thereof
  • Infusion cell freezing medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 can infuse cell cryopreservation solution

[0031] This embodiment provides an infusionable cell cryopreservation solution, the components of which include by volume percentage: glycerin 10v / v%, human albumin injection 20v / v%, hydroxyethyl starch 40 sodium chloride injection 62.2 v / v%, dextran 40 amino acid injection 3.6v / v%, BCG polysaccharide nucleic acid injection 0.5v / v%, and compound electrolyte injection 3.7v / v%.

Embodiment 2

[0032] Embodiment 2 can infuse cell cryopreservation solution

[0033] This embodiment provides an infusible cell cryopreservation solution, the components of which include by volume percentage: glycerin 10v / v%, human albumin injection 20v / v%, and hydroxyethyl starch 40 sodium chloride injection 70v / v%.

Embodiment 3

[0034] Embodiment 3 can transfuse cell cryopreservation solution

[0035] This embodiment provides an infusionable cell cryopreservation solution, the components of which include by volume percentage: glycerin 10v / v%, human albumin injection 20v / v%, hydroxyethyl starch 40% and sodium chloride injection 60v / v%, and BCG polysaccharide nucleic acid injection 10v / v%.

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Abstract

The invention provides an infusion cell cryopreservation solution. The infusion cell cryopreservation solution comprises the following components in percentage by volume: 10% of glycerol; 20% of a human serum albumin injection; and the balance of hydroxyethyl starch 40 sodium chloride injection. Wherein the human serum albumin injection contains human serum albumin with the mass ratio of 20%; the hydroxyethyl starch 40 sodium chloride injection comprises 60g / L of hydroxyethyl starch 40 and 9g / L of sodium chloride. The cell freezing medium capable of being infused does not contain dimethyl sulfoxide, clinical adverse reactions caused by dimethyl sulfoxide in a traditional cell freezing medium are avoided, and efficient, safe and stable cell freezing is achieved; in addition, the cell cryopreservation liquid capable of being infused can be used for clinical-grade cryopreservation of CAR-NK cells and can be directly infused after resuscitation.

Description

technical field [0001] This application relates to the technical field of cells and cell engineering, in particular to an infusible cell cryopreservation solution for clinical-grade cryopreservation of CAR-NK cells. Background technique [0002] Immune cell therapy refers to a treatment method in which the patient's own immature immune cells are activated, cultured, proliferated in vitro, and then reinfused into the body to induce an autoimmune response. The cells currently used in immune cell therapy mainly include T cells, NK cells, CIK cells, etc. Among them, NK cells are more likely to become off-the-shelf products because they do not require strict HLA matching and do not carry the risk of graft-versus-host disease , can be frozen for long-term use. [0003] CAR-NK cell technology refers to the use of scFv antibody fragments that can recognize specific tumor antigens through genetic engineering methods to transform NK cells into CAR-NK cells that can specifically attac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 蔡海波石晓迪黄慧敏谭文松
Owner EAST CHINA UNIV OF SCI & TECH
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