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Detection method of ganoderma lucidum spore powder

A technology of Ganoderma lucidum spore powder and detection method, which can be applied to measuring devices, instruments, scientific instruments, etc., can solve problems such as confusion, uneven product quality, and no quality control system for Ganoderma lucidum spore powder, so as to achieve good quality judgment and improve accuracy. Effect

Pending Publication Date: 2022-03-25
名大生物科技(武汉)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies in the prior art, the present invention provides a detection method for Ganoderma lucidum spore powder, which solves the problem that there are various Ganoderma lucidum spore powders on the market, and thus the quality of its products is uneven and confusing, and there is still no complete method at present. Questions about the quality control system of Ganoderma lucidum spore powder

Method used

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  • Detection method of ganoderma lucidum spore powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A detection method of Ganoderma lucidum spore powder, comprising the following steps:

[0028] S1. Preparation of control solution: Weigh 0.1 g of anhydrous glucose dried at 100°C to constant weight, put it into a measuring cup after weighing it, add water to dissolve and dilute it, and shake it well. For the control solution;

[0029] S2. Preparation of anthrone sulfate solution: Weigh 0.1 g of anthrone, add 80 ml of 74% sulfuric acid solution, dissolve and dilute it, and shake well to obtain anthrone sulfate solution;

[0030] S3. Preparation of test sample: Weigh 3g of Ganoderma lucidum spore powder, put it into a measuring cup after weighing, add 70ml of water to dilute and shake well, then heat it for 5h, after heating, filter it, filter Finally, take by weighing 10ml of filtrate, and add 110ml of absolute ethanol to the filtrate, dilute it and shake it up, then add 20ml of water to the residue to dilute and shake it up, pour it into the filtrate after shaking up;...

Embodiment 2

[0042] A detection method of Ganoderma lucidum spore powder, comprising the following steps:

[0043] S1. Preparation of control solution: Weigh 0.3g of anhydrous glucose dried at 110°C to constant weight, put it into a measuring cup after weighing it, add water to dissolve and dilute it, and shake it well. For the control solution;

[0044] S2. Preparation of anthrone sulfate solution: Weigh 0.3 g of anthrone, add 90 ml of 78% sulfuric acid solution, dissolve and dilute it, and shake well to obtain anthrone sulfate solution;

[0045] S3. Preparation of the test sample: Weigh 4g of Ganoderma lucidum spore powder, put it into a measuring cup after weighing, add 80ml of water to dilute and shake well, then heat it for 6h, after the heating is completed, filter it, filter Finally, take by weighing 10ml of filtrate, and add 140ml of absolute ethanol to the filtrate, dilute and shake it up, then add 25ml of water to the residue, dilute and shake it up, pour it into the filtrate af...

Embodiment 3

[0057] A detection method of Ganoderma lucidum spore powder, comprising the following steps:

[0058] S1. Preparation of control solution: Weigh 0.2g of anhydrous glucose dried at 105°C to constant weight, put it into a measuring cup after weighing it, add water to dissolve and dilute it, and shake it well. For the control solution;

[0059] S2. Preparation of anthrone sulfate solution: Weigh 0.2 g of anthrone, add 85 ml of 76% sulfuric acid solution, dissolve and dilute it, and shake well to obtain anthrone sulfate solution;

[0060] S3. Preparation of the test sample: Weigh 3.5g of Ganoderma lucidum spore powder, put it into a measuring cup, add 75ml of water to dilute and shake well, then heat it for 5.5h, and filter it after heating After filtering, take by weighing 9ml of filtrate, and add 120ml of absolute ethanol to the filtrate, dilute and shake it, then add 23ml of water to the residue, dilute and shake, pour into the filtrate after shaking;

[0061] S4. Curve drawi...

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Abstract

The invention discloses a detection method of ganoderma lucidum spore powder, relates to the technical field of ganoderma lucidum spore powder, and aims to solve the problems that the ganoderma lucidum spore powder in the market at present is various, so that the quality of the ganoderma lucidum spore powder is uneven and disordered, and a complete quality control system related to the ganoderma lucidum spore powder still does not exist at present. Comprising the following steps: preparing a contrast solution, preparing an anthranone sulfate solution, preparing a test sample, drawing a curve, determining a sample, repeatedly determining, weighing 0.1 g of anhydrous dextrose which is dried to constant weight at 100 DEG C, putting the weighed anhydrous dextrose into a measuring cup, adding water for dissolving and diluting, and uniformly shaking to obtain the contrast solution after uniformly shaking. According to the detection method of the ganoderma lucidum spore powder, the intrinsic quality of the ganoderma lucidum spore powder is researched and detected by adopting a thin-layer chromatography-spectrophotometric method, the quality standard of the ganoderma lucidum spore powder is formulated, an experimental basis is provided for the ganoderma lucidum spore powder, and then whether the quality of the ganoderma lucidum spore powder reaches the standard or not is well judged.

Description

technical field [0001] The invention relates to the technical field of ganoderma lucidum spore powder, in particular to a detection method for ganoderma lucidum spore powder. Background technique [0002] Ganoderma lucidum spore powder is a large number of reproductive cells produced by the growth and maturity of Ganoderma lucidum. Its chemical components are mainly proteins, amino acids, vitamins, carotene, alkaloids, fatty acids, and rich polysaccharides, oligosaccharides, and living substances. The main function of Ganoderma lucidum spore powder is to improve the immunity of the human body, and it has a better effect in regulating immunity. [0003] But the Ganoderma lucidum spore powder on the market is varied at present, thus the quality of its products is mixed, relatively confused, and there is still no complete quality control system about the Ganoderma lucidum spore powder at present. Contents of the invention [0004] Aiming at the deficiencies of the prior art,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/74G01N30/06
CPCG01N30/90G01N30/74G01N30/06
Inventor 葛建敏王燕勤葛靖
Owner 名大生物科技(武汉)有限公司
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