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Myocardial infarction related chromosome outer circular DNA as well as screening method and application thereof

A kind of myocardial infarction and extrachromosomal technology, applied in the field of biomedicine, can solve problems such as unreported

Pending Publication Date: 2022-03-25
THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV
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  • Abstract
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Problems solved by technology

Sin STK et al. (Sin STK, JiangP, Deng J, et al. Identification and characterization of extrachromosomal circular DNA in maternal plasma. Proc Natl Acad Sci U S A. 2020; 117(3): 1658-1665. doi: 10.1073 / pnas. 1914949117) showed that maternal and fetal cell-free circular DNA can be isolated from plasma of pregnant women, Sun Z et al. (Sun Z, Ji N, Zhao R, Liang J, Jiang J, TianH.Extrachromosomal circular DNAs are common and functional in esophageal squamous cell carcinoma.Ann Transl Med.2021;9(18):1464.doi:10.21037 / atm-21-4372) revealed the eccDNA expression profile in esophageal squamous cell carcinoma and explored its underlying mechanism, However, extrachromosomal circular DNA related to myocardial infarction has not been reported

Method used

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  • Myocardial infarction related chromosome outer circular DNA as well as screening method and application thereof
  • Myocardial infarction related chromosome outer circular DNA as well as screening method and application thereof
  • Myocardial infarction related chromosome outer circular DNA as well as screening method and application thereof

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Experimental program
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Embodiment

[0030] Concrete implementation process of the present invention is as follows:

[0031] 1. Sample collection and processing

[0032] The samples of the myocardial infarction group were all collected from inpatients in the Department of Cardiology, the Second Affiliated Hospital of Soochow University. The diagnosis of myocardial infarction was in accordance with the "2017 European Guidelines for the Diagnosis and Treatment of Acute ST-segment Elevation Myocardial Infarction". The samples of the control group were from the Second Affiliated Hospital of Soochow University. The peripheral blood of the healthy population in the physical examination center, the myocardial infarction group and the control group each include five subjects, all of which are male and younger than 35 years old.

[0033] Use an anticoagulant tube to collect whole blood, and centrifuge the plasma as soon as possible: centrifuge the sample at 3000g at 4°C for 10 minutes to separate the plasma and cell compo...

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Abstract

The invention provides myocardial infarction related chromosome outer circular DNA as well as a screening method and application thereof. The expression of the screened myocardial infarction related chromosome outer circular DNA in the plasma of a myocardial infarction patient is obviously increased, and the molecular mechanism of myocardial infarction can be better understood by researching the function of the myocardial infarction related chromosome outer circular DNA. The myocardial infarction related chromosome outer circular DNA can be used as a potential biomarker for diagnosis, treatment and prognosis of myocardial infarction.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to extrachromosomal circular DNA related to myocardial infarction and its screening method and application. Background technique [0002] Extrachromosomal circular DNA (eccDNA) widely exists in various eukaryotic organisms including humans, and is closely related to the occurrence and development of many diseases. Sin STK et al. (Sin STK, JiangP, Deng J, et al. Identification and characterization of extrachromosomal circular DNA in maternal plasma. Proc Natl Acad Sci U S A. 2020; 117(3): 1658-1665. doi: 10.1073 / pnas. 1914949117) showed that maternal and fetal cell-free circular DNA can be isolated from plasma of pregnant women, Sun Z et al. (Sun Z, Ji N, Zhao R, Liang J, Jiang J, TianH.Extrachromosomal circular DNAs are common and functional in esophageal squamous cell carcinoma.Ann Transl Med.2021;9(18):1464.doi:10.21037 / atm-21-4372) revealed the eccDNA expression profile in eso...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/11C12Q1/6809C12Q1/6883C12Q1/6858
CPCC12N15/113C12Q1/6809C12Q1/6883C12Q1/6858C12N2310/532C12Q2600/118C12Q2600/158C12Q2600/178C12Q2535/122C12Q2531/113C12Q2535/101C12Q2565/125C12Q2537/165C12Q2539/103C12Q2563/107C12Q2521/107
Inventor 周祥赵诣恒
Owner THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV
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