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Application of lactobacillus rhamnosus LRX-01

A Lactobacillus rhamnosus, mammalian technology, applied in the biological field to achieve the effect of prolonging survival time and reducing intestinal damage

Active Publication Date: 2022-03-25
佛山市孛特碧欧微生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But Lactobacillus rhamnosus has not been reported in the prevention and treatment of diseases caused by high temperature

Method used

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  • Application of lactobacillus rhamnosus LRX-01
  • Application of lactobacillus rhamnosus LRX-01

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Isolation and Identification of Lactobacillus rhamnosus LRX-01

[0036] 1. Isolation of Lactobacillus rhamnosus LRX-01

[0037] Include the following steps:

[0038] (1) Collect the fresh feces of healthy newborns who are exclusively breastfed, and add PBS to dilute according to the solid-liquid ratio of 1:1000 (g / ml);

[0039] (2) Take 5 μl of the diluted sample and spread it on the MRS solid medium, and culture it anaerobically at a constant temperature of 37°C for 48 hours;

[0040] (3), according to the color, size, edge shape, etc. of the colony, use the inoculation loop to pick the colony, and purify by streaking;

[0041] (4) Pick a single colony, inoculate it into the MRS liquid medium, and enrich the bacteria for culture.

[0042] 2. Morphological characteristics of the strain

[0043] Colony characteristics: The colony grows well, is milky white, round, raised, with neat edges.

[0044] Bacterial characteristics: short rod-shaped, immobile, non-sp...

Embodiment 2

[0061] Example 2 The tolerance test of Lactobacillus rhamnosus LRX-01 of the present invention to artificial gastric juice

[0062] Prepare artificial gastric juice with pH value of 2.0, 3.0, 4.0 respectively. Get Lactobacillus rhamnosus standard strain ATCC53103 and Lactobacillus rhamnosus LRX-01 of the present invention respectively, dilute to 10 with sterile PBS 9 CFU / ml bacterial suspension. Pipette 10 μl of the bacterial suspension into 990 μl of artificial gastric juice with different pH values, incubate anaerobically at 37°C for 1h to 3h, and use the dilution drop plate method to determine the number of viable bacteria initially and after culture. The results are shown in Table 2.

[0063] Table 2 Comparison of resistance to artificial gastric juice between ATCC53103 and LRX-01

[0064] strain pH2 pH3 pH4 ATCC53103 1.30％±0.14％ 94.80％±4.09％ 96.48％±2.73％ LRX-01 2.09％±0.07％ 97.35％±2.51％ 97.37％±4.05％

[0065] It can be seen from Table...

Embodiment 3

[0066] Embodiment 3 Lactobacillus rhamnosus LRX-01 of the present invention produces short-chain fatty acid ability test

[0067] Prepare the solutions to be tested as follows:

[0068] (1), transfer to OD 600 Add 100μl of Lactobacillus rhamnosus LRX-01 bacterial solution of =1 to 8ml MRS broth medium and cultivate for 24h, take 1mL of the bacterial suspension in a 1.5mL centrifuge tube, centrifuge at 10000rpm / min for 2min, take 100μl of the supernatant, and add it to 5μl Standard (IS), 5 μl deionized water, 150 μl methanol, 40 μl 2.5% H 2 SO 4 , 0.05g of anhydrous sodium sulfate, vortexed for 1.5min, and centrifuged (14000rpm, room temperature, 5min); the supernatant was taken into a vial equipped with a liner for testing.

[0069] (2), transfer to OD 600 =1, add 100 μl of Lactobacillus rhamnosus standard strain ATCC53103 bacteria solution to 8ml MRS broth medium and cultivate for 24 hours, take 1mL of bacterial suspension in a 1.5mL centrifuge tube, centrifuge at 10000rp...

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Abstract

The invention discloses an application of Lactobacillus rhamnosum LRX-01 in preparation of a product for preventing and treating human or mammal diseases caused by high temperature. When a heat stress test is carried out on a mouse, the inventor of the invention finds that the survival time of the mouse subjected to high-temperature treatment can be obviously prolonged by pretreating the mouse with the Lactobacillus rhamnosum LRX-01, and the survival time of the mouse subjected to high-temperature treatment can be obviously prolonged by treating the mouse with the Lactobacillus rhamnosum LRX-01, so that the survival time of the mouse subjected to high-temperature treatment can be obviously prolonged, and the application of the Lactobacillus rhamnosum LRX-01 in preparation of the product for preventing and treating the human or mammal diseases caused by high temperature can be obviously prolonged. The lactobacillus rhamnosus LRX-01 has the advantages that the lactobacillus rhamnosus LRX-01 can be used for preventing and treating human or mammal diseases caused by high temperature, the serum inflammatory factors and endotoxin of the lactobacillus rhamnosus LRX-01 can be reduced, the intestinal injury caused by heat stress can be relieved, and the results show that the lactobacillus rhamnosus LRX-01 has potential application value in the aspect of preventing and treating the human or mammal diseases caused by high temperature.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of a strain of Lactobacillus rhamnosus LRX-01 in the preparation of products for preventing and treating human or mammalian diseases caused by high temperature. Background technique [0002] High temperature can lead to heat stress (heat stress, HS), heat stroke (heatstroke) or psychotic neurological disorders. HS is a stress response experienced by the body in a high-temperature environment. When the rate of heat gain in the body is higher than the rate of heat dissipation, hyperthermia will occur. When the body temperature rises to a critical level of 40.5°C, it will cause heat stroke. It is characterized by Central nervous system damage and multiorgan failure. Heat stroke has become a life-threatening health problem as the number of deaths from heat stroke begins to rise due to rising global temperatures, expanding urban environments, and increasing li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P39/00A61P29/00A61P25/00A61P1/00A23L33/135A23K10/18A23K10/12A23L33/00A23L19/00A23L2/38A23L2/52A23C9/123A23C9/152C12R1/225
CPCC12N1/20A61K35/747A61P39/00A61P29/00A61P25/00A61P1/00A23L33/135A23K10/18A23K10/12A23L33/00A23L19/00A23L2/382A23L2/52A23C9/1234A23C9/152A23V2002/00A23V2400/175A23V2200/30A23V2200/322Y02A50/30
Inventor 孟晓静范宏英曾位森张宏男温俊杰陈振辉
Owner 佛山市孛特碧欧微生物科技有限公司
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