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Mulberry leaf extract rich in 1-deoxynojirimycin and preparation method thereof

The technology of deoxynojirimycin and mulberry leaf extract is applied in the directions of medical preparations, drug combinations, and pharmaceutical formulations containing active ingredients, and can solve the problems of low efficacy, low extraction efficiency of 1-deoxynojirimycin, and the like, To achieve the effect of improving yield, convenient edibility, and overcoming the color black and green

Active Publication Date: 2022-03-15
湖北瑞邦生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] Aiming at the deficiencies in the prior art, the invention provides a mulberry leaf extract rich in 1-deoxynojirimycin and a preparation method thereof, which The problem of low extraction efficiency and low efficacy of 1-deoxynojirimycin existing in the prior art is solved

Method used

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  • Mulberry leaf extract rich in 1-deoxynojirimycin and preparation method thereof
  • Mulberry leaf extract rich in 1-deoxynojirimycin and preparation method thereof
  • Mulberry leaf extract rich in 1-deoxynojirimycin and preparation method thereof

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Experimental program
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Embodiment 1

[0045] The preparation of embodiment 1 mulberry leaf extract

[0046] 1) washing, drying and pulverizing the harvested fresh mulberry leaves to obtain mulberry leaf powder;

[0047] 2) Add 15 times the mass of water, at about 70°C, ultrasonic for 2 hours; use a tube centrifuge for 20 minutes at a speed of 20,000r / min to obtain the supernatant and residue, and continue centrifuging to obtain the supernatant and residue. The supernatants obtained twice were combined to obtain supernatant 1;

[0048] 3) Add water of equal quality to the residue obtained in step 2), adjust the pH value of the feed liquid to 4.5, add compound enzyme 1 with a residue mass of 0.2%, maintain the system temperature at 55° C. and pH 4.5 during the hydrolysis process, and the time is 3h; adjust the pH to 7.8, add compound enzyme 2 with a residue mass of 0.2%, and continue the enzymolysis for 3h at a temperature of 55°C; centrifuge the enzymolysis solution obtained in step 3) for 10min with a tube centri...

Embodiment 2

[0054] The preparation of embodiment 2 mulberry leaf extract

[0055] 1) washing, drying and pulverizing the harvested fresh mulberry leaves to obtain mulberry leaf powder;

[0056] 2) Add 10 times the mass of water, at about 55°C, ultrasonic for 3 hours; use a tube centrifuge to centrifuge for 15 minutes at a speed of 25000r / min to obtain the supernatant and residue, and continue centrifuging to obtain the supernatant and residue. The supernatants obtained twice were combined to obtain supernatant 1;

[0057] 3) Add equal mass of water to the residue obtained in step 2), adjust the pH value of the feed solution to 4, add compound enzyme 1 with a residue mass of 0.22%, and maintain the system temperature at 50° C. and pH 4 during the hydrolysis process for 2 hours; Adjust the pH to 8.5, add compound enzyme 2 with a residue mass of 0.22%, and continue the enzymolysis for 2 hours at a temperature of 60°C; centrifuge the enzymolysis solution obtained in step 3) for 15 minutes wi...

Embodiment 3

[0063] The preparation of embodiment 3 mulberry leaf extract

[0064] 1) Washing, drying and pulverizing the harvested fresh mulberry leaves,

[0065] 2) Add 30 times the mass of water, at about 75°C, ultrasonic for 1 hour; use a tube centrifuge for 10 minutes at a speed of 30,000r / min to obtain supernatant and residue, and continue centrifuging to obtain supernatant and residue. The supernatants obtained twice were combined to obtain supernatant 1;

[0066] 3) Add water of equal quality to the residue obtained in step 2), adjust the pH value of the feed solution to 5, add compound enzyme 1 with a residue mass of 0.18%, and maintain the system temperature at 53° C. and pH 5 during the hydrolysis process for 4 hours; Adjust the pH to 8, add compound enzyme 2 with a residue mass of 0.18%, and continue the enzymolysis for 4 hours at a temperature of 50°C; centrifuge the enzymolysis solution obtained in step 3) for 20 minutes at a speed of 25,000 r / min at a temperature of 20°C, ...

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Abstract

The invention provides a mulberry leaf extract rich in 1-deoxynojirimycin and a preparation method of the mulberry leaf extract. The preparation method comprises the following steps: S1, pretreatment: cleaning, drying and crushing mulberry leaves to obtain mulberry leaf powder; s2, ultrasonic treatment: adding the mulberry leaf powder into water, performing ultrasonic treatment and centrifugation, and taking supernate to obtain supernate 1 and residues; s3, performing enzymolysis treatment: adding water with the same mass as the residues, performing enzymolysis by using a compound enzyme 1, performing enzymolysis by using a compound enzyme 2, performing centrifugation, and taking supernate to obtain supernate 2; and S4, filtering, concentrating and drying: combining the supernatant 1 in the S2 and the supernatant 2 in the S3, and carrying out membrane filtration, membrane concentration and spray drying. The mulberry leaf extract obtained by the method is high in extraction rate, retains active ingredients in mulberry leaves to a great extent, has an extremely strong blood sugar reducing effect, is safe, good in quality and light in color, and has plant faint scent.

Description

technical field [0001] The invention relates to the technical field of mulberry leaf extraction, in particular to a mulberry leaf extract rich in 1-deoxynojirimycin and a preparation method thereof. Background technique [0002] Mulberry leaves are the leaves of the Moraceae plant mulberry, which are ovate or broadly ovate, about 15 cm long, 10 cm wide, petioles about 4 cm long, heart-shaped at the base, slightly pointed at the top, serrated at the edge, densely white veins soft hair. Older leaves are thicker yellow-green. Young leaves are thin, dark green. The quality is crisp and fragile, and it is difficult to hold. It is produced in most parts of the country, especially in the middle and lower reaches of the Yangtze River and the mulberry area of ​​the Sichuan Basin. Bitter in the mouth, sweet, cold in nature. Return lung, Liver Channel. Efficacy Evacuation of wind-heat, clearing the liver and improving eyesight. Indications for wind-heat cold, cough, headache, re...

Claims

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Application Information

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IPC IPC(8): A61K36/605A61K31/445A61P3/10A61K127/00
CPCA61K36/605A61K31/445A61P3/10A61K2236/19A61K2236/51A61K2236/53
Inventor 王彩霞王希搏王微尹晓清靳帧亮伍梦婷薛丽丽
Owner 湖北瑞邦生物科技有限公司
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