Method for detecting toxoflavin and hotchenkine in food and degradation products of toxoflavin and hotchenkine

Abstract

The invention relates to the field of food safety, in particular to a method for detecting toxoflavin, homeochin and degradation products of toxoflavin and homeochin in food. The method comprises the following steps: treating a sample, setting instrument conditions, demonstrating feasibility of a detection method, detecting toxoflavin and hotchenkine in the sample, simulating degradation products of toxoflavin and hotchenkine under different conditions and deducing molecular weight, and detecting the degradation products of toxoflavin and hotchenkine in the sample. The method for detecting the toxoflavin and the hotchenkine as well as the degradation products of the toxoflavin and the hotchenkine in the food is simple in pretreatment, accurate in qualitative analysis and high in specificity, and the toxoflavin and the hotchenkine as well as the degradation products of the toxoflavin and the hotchenkine in the food can be rapidly and quantitatively analyzed within ten minutes.

Description

technical field [0001] The invention belongs to the field of food safety, and in particular relates to a method for detecting food poisoning flavin, zemycin and degradation products thereof. Background technique [0002] Toxoflavin (toxoflavin, CAS number: 84-82-2), molecular weight C 7 h 7 N 5 o 2 , Toxicology shows: the LD50 of toxin in mice is 1.7mg / kg by intravenous injection, and 8.39mg / kg by oral administration, which has strong toxicity. Fervenulin (CAS No.: 483-57-8), molecular weight C 7 h 7 N 5 o 2 , is the isomer of toflavin, under certain chemical conditions, the two can be transformed into each other. Its toxicology shows that the LD50 of zeremycin hamster intraperitoneal injection is 11.2 mg / kg, and it also has strong toxicity. The structural formulas of toflavin and zeremycin are as follows: [0003] [0004] In October 2020, an incident of poisoning from eating "fermented rice noodles" occurred in Jixi, Heilongjiang. In June 1984, food poisoning ...

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Problems solved by technology

[0005] Because toflavin can be converted into zeremycin under certain conditions, and is unstable and prone to degradation under alkaline and oxidative conditions, the prior art only has high performance liquid chromatography for simultaneously detecting toflavin and zeirecin. There is a lot of interference during the measurement, and false positives may occur, resulting in misjudgment of late diagnosis. The analysis time is long, which is not conducive to the rapid and accurate qualitative requirements of sudden poisoning events, and cannot identify its degradation products, and cannot determine whether food has been poisoned. Contaminated by flavin and zemycin, which will affect the later treatment

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