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Epidermal stem cell in-vitro culture kit and application thereof

An epidermal stem cell and in vitro culture technology, which is applied in the field of epidermal stem cell in vitro culture kits, can solve the problems of high operating level requirements, unfavorable large-scale preparation, complicated operation, etc., and achieves broad application prospects, good transformation efficiency, and high purity. Effect

Pending Publication Date: 2022-03-11
深圳市申友健康管理有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current conventional method is to obtain epidermal stem cells by primary culture of epidermal tissue, but this method is complicated to operate and requires cell sorting, which requires a high level of technical personnel; the obtained epidermal stem cells need to use feeder cells when culturing , high cost, not conducive to large-scale preparation

Method used

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  • Epidermal stem cell in-vitro culture kit and application thereof

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Comparison scheme
Effect test

Embodiment 1

[0064] This embodiment provides a kit for in vitro culture of epidermal stem cells, the kit for in vitro culture of epidermal stem cells includes a medium for promoting adhesion and a medium for promoting transformation;

[0065] In terms of volume fraction, the medium for promoting adhesion includes 8% cell culture supplement, 8% fetal bovine serum, rhGM-CSF 1000IU / mL, bFGF 100ng / mL, SCF 100ng / mL and IL-3 100ng / mL, The balance is stem cell culture medium;

[0066] In terms of volume fraction, the transformation-promoting medium includes cell culture supplement 8%, EGF 70IU / mL, BPE 70μg / mL, fibronectin 70μg / mL and IL-3 70ng / mL, and the balance is stem cell medium.

Embodiment 2

[0068] This embodiment provides a kit for in vitro culture of epidermal stem cells, the kit for in vitro culture of epidermal stem cells includes a medium for promoting adhesion and a medium for promoting transformation;

[0069] In terms of volume fraction, the medium for promoting adhesion includes 10% cell culture supplement, 10% fetal calf serum, rhGM-CSF 800IU / mL, bFGF 80ng / mL, SCF 80ng / mL and IL-3 80ng / mL, The balance is stem cell culture medium;

[0070] In terms of volume fraction, the transformation-promoting medium includes cell culture supplement 10%, EGF 40IU / mL, BPE40μg / mL, fibronectin 40μg / mL and IL-3 40ng / mL, and the balance is stem cell medium.

Embodiment 3

[0072] This embodiment provides a kit for in vitro culture of epidermal stem cells, the kit for in vitro culture of epidermal stem cells includes a medium for promoting adhesion and a medium for promoting transformation;

[0073] In terms of volume fraction, the medium for promoting adhesion includes 1% cell culture supplement, 1% fetal calf serum, rhGM-CSF 1200IU / mL, bFGF 120ng / mL, SCF 120ng / mL and IL-3 120ng / mL, The balance is stem cell culture medium;

[0074] In terms of volume fraction, the transformation-promoting medium includes cell culture supplement 1%, EGF 80IU / mL, BPE 80μg / mL, fibronectin 80μg / mL and IL-3 80ng / mL, and the balance is stem cell medium.

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Abstract

The invention provides an epidermal stem cell in-vitro culture kit and application thereof. The epidermal stem cell in-vitro culture kit comprises an adherence-promoting culture medium and a transformation-promoting culture medium, the adherence promoting culture medium comprises a stem cell culture medium, a cell culture supplement, fetal calf serum, adherence promoting cell factors and IL-3, and the concentration of IL-3 is 80-120 ng / mL; the transformation-promoting culture medium comprises a stem cell culture medium, a cell culture supplement, a transformation-promoting cell factor, fibronectin and IL-3, and the concentration of IL-3 is 40-80 ng / mL. The invention also provides an epidermal stem cell in-vitro culture method, an epidermal stem cell and an anti-wrinkle injection. The optimized culture medium is matched with the culture method, a large number of epidermal stem cells with high purity can be rapidly obtained, and the epidermal stem cells serving as components of the anti-wrinkle injection are obvious in effect and high in value.

Description

technical field [0001] The invention belongs to the technical field of cell biology, and in particular relates to a kit for culturing epidermal stem cells in vitro and an application thereof. Background technique [0002] Epidermal stem cells (ESCs) are a type of cell population that has the ability to proliferate and can proliferate and differentiate into various functional cells in the epidermis. It is mainly located in the basal layer of the epidermis, belongs to adult stem cells, has a strong ability to divide and proliferate, and can replenish aging and exfoliated keratinocytes. [0003] Epidermal stem cells, as skin tissue-specific stem cells, not only maintain the daily metabolism of the epidermis, but also actively participate in the repair of skin after damage. It is of great significance to explore the proliferation, differentiation and migration of epidermal stem cells. Therefore, obtaining and culturing epidermal stem cells in vitro is the basis of related resea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
CPCC12N5/0625C12N2501/2303C12N2533/52C12N2501/22C12N2501/115C12N2501/125
Inventor 易亮梁秋彬罗国辉钱春明颜春江汪金球黄景云邵小兵褚志伟吴佑星
Owner 深圳市申友健康管理有限公司
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