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Microbial preparation for preventing and treating plant diseases as well as preparation method and application of microbial preparation

A technology for microbial preparations and plant diseases, applied in the field of microbial preparations for preventing and treating plant diseases and their preparation, can solve problems such as difficulty in achieving control effects, and achieve the effects of significant control effects, enhanced control effects, and reduced morbidity rates.

Pending Publication Date: 2022-03-11
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the biocontrol bacteria preparations are fermentation preparations of single biocontrol bacteria; however, it is often difficult for a single biocontrol bacteria to achieve the desired control effect

Method used

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  • Microbial preparation for preventing and treating plant diseases as well as preparation method and application of microbial preparation
  • Microbial preparation for preventing and treating plant diseases as well as preparation method and application of microbial preparation
  • Microbial preparation for preventing and treating plant diseases as well as preparation method and application of microbial preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The present embodiment provides a kind of microbial preparation for preventing and treating plant diseases, and its formula is:

[0031] 10 parts of Bacillus Velez HAB-2, 10 parts of Trichoderma harzianum, 5 parts of Beauveria bassiana, 3 parts of Metarhizium anisopliae;

[0032] Among them, the preservation number of Bacillus Velez HAB-2 is: CCTCC NO: M 2015070, and the total strain concentration in the microbial preparation is 10 9 CFU / mL.

[0033] Its preparation method is:

[0034] Take Bacillus Velez HAB-2, Trichoderma harzianum, Beauveria bassiana and Metarhizium anisopliae, respectively inoculate them into PDA medium, cultivate and ferment them, and then mix them to obtain the product.

Embodiment 2

[0036] The present embodiment provides a kind of microbial preparation for preventing and treating plant diseases, and its formula is:

[0037] 20 parts of Bacillus Velez HAB-2, 5 parts of Trichoderma harzianum, 1 part of Beauveria bassiana, 1 part of Metarhizium anisopliae;

[0038] Among them, the preservation number of Bacillus Velez HAB-2 is: CCTCC NO: M 2015070, and the total strain concentration in the microbial preparation is 10 9 CFU / mL.

[0039] Its preparation method is:

[0040] Take Bacillus Velez HAB-2, Trichoderma harzianum, Beauveria bassiana and Metarhizium anisopliae, respectively inoculate them into PDA medium, cultivate and ferment them, and then mix them to obtain the product.

Embodiment 3

[0042] The present embodiment provides a kind of microbial preparation for preventing and treating plant diseases, and its formula is:

[0043] 12 parts of Bacillus Velez HAB-2, 8 parts of Trichoderma harzianum, 3 parts of Beauveria bassiana, 2 parts of Metarhizium anisopliae;

[0044] Among them, the preservation number of Bacillus Velez HAB-2 is: CCTCC NO: M 2015070, and the total strain concentration in the microbial preparation is 10 9 CFU / mL.

[0045] Its preparation method is:

[0046] Take Bacillus Velez HAB-2, Trichoderma harzianum, Beauveria bassiana and Metarhizium anisopliae, respectively inoculate them into PDA medium, cultivate and ferment them, and then mix them to obtain the product.

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PUM

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Abstract

The invention discloses a microbial preparation for preventing and treating plant diseases, which is characterized by comprising the following microbial preparations in parts by weight: 10-20 parts of bacillus velezensis HAB-2, 5-10 parts of trichoderma harzianum, 1-5 parts of beauveria bassiana and 1-3 parts of metarhizium anisopliae. Wherein the preservation number of the bacillus velezensis HAB-2 is CCTCC (China Center For Type Culture Collection) NO: M 2015070. Correspondingly, the invention further provides a preparation method and application of the microbial preparation. The microbial preparation disclosed by the invention has remarkable prevention and treatment effects on areca-nut anthracnose, guava leaf blight, navel orange anthracnose, longan leaf spot disease, wax apple fruit rot and the like, and can effectively reduce the morbidity.

Description

technical field [0001] The invention relates to the field of microbial preparations, in particular to a microbial preparation for preventing and treating plant diseases, a preparation method and application thereof. Background technique [0002] The use of biocontrol bacteria to control plant diseases and insect pests has been more and more applied to actual production. At present, most bio-control bacteria preparations are fermented preparations of single bio-control bacteria; however, it is often difficult for a single bio-control bacteria to achieve the desired control effect. [0003] On the other hand, the inventor has found a strain of Bacillus amyloliquefaciens HAB-2 (CN201510061973.0) in previous research; Preliminary research shows that it is effective for rubber brown root disease (Phellinus noxius (Corner) G.H.Cunn. ), tropical plant pathogens have good antibacterial effect. Further studies have shown that after mixed fermentation with Bacillus subtilis, Trichod...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/20A01N63/22A01N63/38A01N63/30A01P3/00C12R1/07C12R1/885C12R1/645
CPCC12N1/14C12N1/20A01N63/22A01N63/38A01N63/30
Inventor 刘文波秦春秀缪卫国林春花李潇李志刚
Owner HAINAN UNIVERSITY
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