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White pleurotus pulmonarius and cultivation method thereof

A lung-shaped Pleurotus and culture technology, which is applied in the cultivation of white pneumophila strains and the field of white pneumococcus strains, can solve the problem of not finding lung-shaped Pleurotus, and achieves high economic benefit, high commodity value and taste for mushroom farmers. crisp effect

Active Publication Date: 2022-03-08
GUIZHOU INST OF SOIL & FERTILIZER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After searching, no lung-shaped Pleurotus with a white cap was found

Method used

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  • White pleurotus pulmonarius and cultivation method thereof
  • White pleurotus pulmonarius and cultivation method thereof
  • White pleurotus pulmonarius and cultivation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 The collection, isolation and domestication process of bacterial strain Qianfang 02 of the present invention

[0039] In August 2019, the inventor discovered wild white edible fungus fruiting bodies on the top of Mount Fanjing in the Fanjing Mountain area of ​​Tongren, Guizhou. Isolate the wild fruiting body tissue to obtain mycelia (bacteria), then carry out the fruiting test of the bacterial species after the tissue separation, and then isolate the fruiting body with good flower shape, inoculate it on the PDA medium for cultivation, The strains with fast mycelial growth and uniform growth were screened, and the best strains continued to be cultivated and domesticated. After several generations of cultivation, a pure white edible fungus strain with stable genetic properties was screened out, named: Qianfang 02.

Embodiment 2

[0040] Example 2 Classification and identification of bacterial strain Qianfang 02 of the present invention

[0041] (1) Morphological identification of Qianfang 02:

[0042]The fruiting body is clustered, pure white; the cap is fan-shaped, the cross section of the cap is flat, the middle part is slightly concave, the diameter of the cap is 5.1-12.3 cm, an average of 8.2 cm; the thickness of the cap is moderate, 0.8-1.2 cm, an average of 1.0 cm; the stipe is long 0.8-2.3cm; gills without reticulation, gills extending to the top of the stipe; fruiting bodies are compact in texture. According to the photographs and corresponding feature descriptions on page 990 of the "Illustrated Book of Large-scale Fungal Resources in China" (edited by Li Yu, Zhongyuan Farmers Publishing House, published in 2015), Qianfang 02 is consistent with the morphological characteristics of Pleurotus lungus, indicating that Qianbian 02 belongs to Pleurotus pulmonarius (Fr.) Quél. in classification.

...

Embodiment 3

[0050] Embodiment 3 The PAPD comparative analysis test (one) of bacterial strain Qian side 02 of the present invention and other Pleurotus pneumophila varieties: 1, Qian side 02; 3. Jingxiu No. 1 (light brown lung-shaped Pleurotus bred in Jijiayuan, Beijing); 4. Xiu 03 (gray lung-shaped Pleurotus from Chuangjian Taijiang, Qiandongnan), 5. Xiaxiu 004 ( Brown lung-shaped Pleurotus bred by Jiangsu Tianlong Fungus).

[0051] (2) Test method

[0052] Genomic DNA of Qianbian 02, Pteris praecox, Jingxiu No. 1, Xiu 03 and Xiaxiu 004 strains were respectively extracted by plant genomic DNA extraction kit CW0531 (Beijing Kangwei Century Company), and 20 RAPD primers were selected (see Table 1 ) for PCR amplification.

[0053] Table 1 RAPD random primer sequence

[0054] Primer name Primer sequence 5'-3' Primer name Primer sequence 5'-3' S18 CCACAGCAGT S80 ACTTCGCCAC S24 AATCGGGCTG S90 AGGGCCGTCT S27 GAAACGGGTG S94 GGATGAGACC S30 GTGATC...

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Abstract

The invention belongs to the field of edible mushrooms, and particularly discloses a Pleurotus pulmonarius (Fr.) Quell bacterial strain Qianside 02, and the preservation number of the Pleurotus pulmonarius (Fr.) Quell bacterial strain is CCTCC (China Center for Type Culture Collection) NO: M2021259. The invention also discloses application of the strain in food, health care products or beverages. The sporocarp of the known pleurotus pulmonarius strain is gray or brown, while the sporocarp of the strain Qianzhou side 02 is pure white and beautiful, and the strain is a novel pleurotus pulmonarius strain type and has extremely high commodity value; secondly, the sporocarp of the strain is compact in texture, crisp in taste and good in eating quality; in addition, the cultivation period of the strain is shortened by 10-20 days compared with that of a known pleurotus pulmonarius strain, and the cultivation period is short; in addition, the yield of 02 on the Qian side is high, and the average yield per bag is 310.7 g. Therefore, the market prospect of the Guizhou side 02 is extremely wide.

Description

technical field [0001] The invention belongs to the field of edible fungi, and in particular relates to a white Pleurotus aeruginosa strain and a cultivation method for the white Pleurotus aeruginosa strain. Background technique [0002] Lung-shaped Pleurotus (also known as Xiuzhen mushroom. Alias: Pleurotus pleurotus, Pleurotus phoenix, Indian oyster mushroom or Himalayan oyster mushroom, etc.; its Latin name: Pleurotus pulmonarius (Fr.) Quél.) belongs to the genus Pleurotus of the family Pleurotus. The shape of Pleurotus pulmonaris is small and beautiful, not only delicate in texture, delicious in taste, but also rich in nutrition, rich in protein, carbohydrates, unsaturated fatty acids, vitamins, folic acid and various trace elements, etc.; Mushrooms are high; its fruiting body contains 18 kinds of amino acids, of which the content of aspartic acid and glutamic acid is relatively high. In addition, the lung-shaped Pleurotus has high protein content and low fatty acid cha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/50A01G18/40A01G18/20
CPCA01G18/00A01G18/50A01G18/40A01G18/20
Inventor 王晓敏杜慕云杨珍陈旭张邦喜朱森林陈波张钦语唐玲杨仁德
Owner GUIZHOU INST OF SOIL & FERTILIZER
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