Experimental method for screening FASN inhibitor in peony seed meal monomeric compound based on computer simulation

Abstract

The invention belongs to the technical field of pharmaceutical analysis, and discloses an experimental method for screening a fatty acid synthase (FASN) inhibitor in a peony seed meal monomeric compound based on computer simulation, and the experimental method for screening the FASN inhibitor in the peony seed meal monomeric compound based on computer simulation comprises the following specific operation steps: S1, molecular docking; s2, cell activity detection; s3, performing protein immunoblotting; and S4, cell apoptosis detection. Whether four monomeric compounds in the peony seed meal extract have anti-tumor activity caused by inhibition of FASN activity and expression or not is screened by means of a molecular docking method, a homologous modeling means is tried to construct a whole enzyme dimer structure of FASN, and a new thought and a new method are provided for screening of the FASN inhibitor. A large number of invalid experiments can be effectively avoided, the success rate of experiment operation is improved, and the experiment operation cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical analysis, in particular to an experimental method for screening FASN inhibitors in peony seed meal monomer compounds based on computer simulation. Background technique [0002] Breast cancer is a common malignant tumor that endangers women's health today. Scientists have been committed to finding ways to eradicate this disease. In recent years, although great progress has been made, the survival rate of breast cancer patients has been greatly improved, but its internal pathogenesis It is still not fully understood, and the method and effectiveness of breast cancer prevention, treatment and prognosis are still not ideal. Since the 1990s, the expression and activity of FASN found in tissue samples such as breast cancer were much higher than normal levels, making it begin to as potential cancer therapeutic targets. [0003] Although many FASN inhibitors have been reported in the past 20 years...

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Problems solved by technology

[0002] Breast cancer is a common malignant tumor that endangers women's health today. Scientists have been committed to finding ways to eradicate this disease. In recent years, although great progress has been made, the survival rate of breast cancer patients has been greatly improved, but its internal pathogenesis It is still not fully understood, and the method and effectiveness of breast cancer prevention, treatment and prognosis are still not ideal. Since the 1990s, the expression and activity of FASN found in tissue samples such as breast cancer were much higher than normal levels, making it begin to as a potential cancer therapeutic target

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