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Application of natural medicine in preparation of antibacterial medicine

A technology of antibacterial drugs and natural drugs, applied in the field of medicine, can solve the problem that the inhibition effect is not much different

Active Publication Date: 2022-03-01
MATERNAL & CHILD HEALTH CARE HOSPITAL OF SHANDONG PROVINCE SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inventors found that some antibacterial preparations of multi-drug-resistant Acinetobacter baumannii in the prior art need to be at least 25 μg / mL to produce inhibitory effects on multi-drug-resistant Acinetobacter baumannii, and with the increase of the concentration of antibacterial preparations, the inhibition The effect is not much different

Method used

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  • Application of natural medicine in preparation of antibacterial medicine
  • Application of natural medicine in preparation of antibacterial medicine
  • Application of natural medicine in preparation of antibacterial medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Static Action Determination of Mangiferin against Multidrug Resistant Acinetobacter baumannii

[0056] 1. Materials

[0057] 1.1 Experimental strains

[0058] During this experiment, the strains (Acinetobacter baumannii AB11, Escherichia coli E1, Klebsiella pneumoniae KP50, Staphylococcus aureus M11) were all obtained from the laboratory department of the hospital, and had been identified as multi-drug resistant strains, and tested Its sensitivity to commonly used clinical antibiotics was shown (Table 1). The strains used in the test were all stored in a -80°C refrigerator, and subcultured on MHA solid medium before use, at least twice.

[0059] Table 1 The results of the sensitivity determination of test strains to commonly used clinical antibiotics

[0060]

[0061]

[0062] 1.2 Drugs and main reagents

[0063] Mangiferin, Dalian Meilun Biotechnology Co., Ltd. (Dalian, Liaoning); amygdalin, Dalian Meilun Biotechnology Co., Ltd. (Dalian, Liaoning); calcium-ad...

Embodiment 2

[0076] The effect of caffeic acid methyl ester against multi-drug resistant Acinetobacter baumannii

[0077] Experimental strain

[0078] The clinically isolated Acinetobacter baumannii (AB2, AB11, AB12) were collected, all strains were correctly identified and their sensitivity to commonly used clinical antibiotics was determined.

[0079] Bacterial suspension preparation

[0080] Pick 3-5 colonies from the MHA plate that has been purified and cultivated overnight, and inoculate them in sterile 0.9% sodium chloride solution. The turbidity of the bacterial solution was adjusted to 0.5 McFarland turbidity (about 1×10 8 CFU / mL), and then diluted 100 times with calcium regulating MH broth medium (about 1×10 6 CFU / mL).

[0081] Caffeic acid methyl ester solution preparation

[0082] According to the requirements of the American Association for Clinical Laboratory Standards standard, the concentration of caffeic acid methyl ester stock solution was prepared to be 40960 μg / mL. ...

Embodiment 3

[0094] Antibacterial effect of dehydrohalampine on enhancing meropenem against Klebsiella pneumoniae

[0095] Experimental strain: Klebsiella pneumoniae

[0096] During this experiment, the strains were all obtained and identified from the laboratory department of the hospital. The strains used in the test were stored in a -80°C refrigerator, and subcultured on MHA solid medium before use, at least twice.

[0097] Drugs and Main Reagents

[0098] Halalline, Dalian Meilun Biotechnology Co., Ltd. (Dalian, Liaoning); Meropenem, Dalian Meilun Biotechnology Co., Ltd. (Dalian, Liaoning); Calcium-adjusted MH Broth Medium (CAMHB Broth), Qingdao High-tech Industry Yuan Haibo Biotechnology Co., Ltd. (Qingdao, Shandong); MHA agar medium, Qingdao High-Tech Industrial Park Haibo Biotechnology Co., Ltd. (Qingdao, Shandong); phosphate buffered saline (PBS), Dalian Meilun Biotechnology Co., Ltd. (Dalian, Liaoning).

[0099] method

[0100] Preparation of culture medium and drug storage solu...

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Abstract

The invention relates to the technical field of medicines, in particular to application of a natural medicine in preparation of antibacterial medicines. According to the invention, various natural medicines can be used for preparing antibacterial medicines; mangiferin has a good inhibition effect on the growth of acinetobacter baumannii; the methyl caffeate has a good inhibition effect on acinetobacter baumannii; the invention also finds that harmine can be used as a synergist of meropenem to enhance the antibacterial effect of meropenem on klebsiella pneumoniae; single use of harmine or meropenem cannot inhibit the growth of klebsiella pneumonia, but when 128 [mu] g / mL harmine and 4 [mu] g / mL meropenem are combined for use, the growth of klebsiella pneumonia can be significantly inhibited, and the growth rate can be reduced to 5% or below.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of a natural medicine in the preparation of antibacterial medicines. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Bacterial drug resistance, also known as drug resistance, refers to the tolerance of bacteria to the action of antibacterial drugs. Once drug resistance occurs, the antibacterial effect of drugs will decrease significantly. When antibiotics are used for a long time, the majority of sensitive strains are constantly killed, and drug-resistant strains multiply in large numbers, replacing sensitive strains, and the rate of bacterial resistance to th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61K31/216A61K31/437A61K31/407A61P31/04
CPCA61K31/352A61K31/216A61K31/437A61K31/407A61P31/04A61K2300/00Y02A50/30
Inventor 李秀云陈兆文蒲兰香韩厚美周玉侠孙敏
Owner MATERNAL & CHILD HEALTH CARE HOSPITAL OF SHANDONG PROVINCE SHANDONG UNIV
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