A rhizoma bletillae seedling growth promoting method based on piriformospora indica
An Indian piriformis, growth-promoting technology, applied in the biological field, can solve the problems that the size of the seedling affects the growth potential of the medicine, affects the germination and growth of the seed, and is easy to pollute.
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[0035] Embodiment 1, adding the growth-promoting effect of Piriformis indica to white and tissue culture seedlings
[0036] (1) Disinfection of the transplanting substrate: after high-temperature sterilization of the seedling-raising substrate and letting it cool (i.e., sterilizing at 121° C. for 60 minutes, and then cooling to room temperature), it is subpackaged in small square pots with a caliber of 7 cm; as the seedling-raising substrate after sterilization, stand-by;
[0037] (2) Preparation of Pyromorpha indica liquid / spore liquid
[0038] ①, the preparation of Pyromorpha indica liquid:
[0039]Cultivate the strain of Pyromorpha indica in a petri dish filled with PDA solid medium, the culture conditions are temperature 25±2℃, photoperiod 12h·d -1 , 10~15 days; On the PDA solid medium, the stainless steel puncher of diameter 6mm is cultivated on the PDA solid medium and is perforated at the edge of the Pyromorpha indica colony, obtains the Pyromorpha indica block contai...
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