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Tissue Culture and Rapid Propagation Method of Kylin Tail Seedlings

A technology for tissue culture rapid propagation and seedlings, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of not being able to obtain a large number of high-quality seedlings, achieve easy multiplication coefficient stability, optimize cultivation conditions, and reduce tissue The effect of browning

Active Publication Date: 2016-03-30
SUNSHINE HORTICULTURE
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AI Technical Summary

Problems solved by technology

[0003] The traditional propagation of Kylin tail is to adopt the method of cutting seedlings, which cannot obtain a large number of high-quality seedlings in a short period of time

Method used

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Embodiment Construction

[0042] In the present invention, the MS improved medium used is to add 220mg.L in the conventional MS medium -1 Ca(NO 3 ) 2 .4H 2 O, and KNO 3 Dosage from 1900mg.L -1 increased to 2100mg.L -1 .

[0043] In the present invention, the peat soil used is provided by Danish Pinshi Company, adopts high-quality peat moss, and the organic matter content reaches 98%.

[0044] The method for tissue culture and rapid propagation of unicorn tail seedlings of the present invention is realized through the following steps:

[0045] 1. Selection and cultivation of explants

[0046] The mother plant is managed according to the routine. From April to October, when the mother plant has 3-5 nodes and the length of the newly germinated terminal bud is about 10-40mm, the explant can be collected. In order to ensure that the regenerated plant has the female parent's For all characters, collect the newly germinated terminal buds or side buds of the mother plant as explants, and the length of ...

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Abstract

The invention discloses a tissue culture and rapid propagation method for epipremnum pinnata seedlings. The tissue culture and rapid propagation method is implemented by four or five steps of selective culture of explants, obtaining of germfree materials, domestication and transgenerational propagation of the germfree materials and strong-seedling rooting culture. According to the tissue culture and rapid propagation method, in the months from April to October, 3-5 knots can be extracted from a stock plant, and a new geminated terminal bud is about 10-40mm long, the new germinated terminal buds or lateral buds of the stock plant, which are 10-20mm long, are collected as the explants, and culture mediums for induction, propagation, strengthening and rooting are improved in an optimized manner; during cutting of transgenerational blocks, thick buds of which the bud thickness is larger than 2.5mm are cut according to the density of 2-4 buds per block, and thin buds of which the bud thickness is smaller than or equal to 2.5mm are cut according to the diameter of the block of 8-10mm; furthermore, culture conditions at all stages of tissue culture seedlings are optimized to guarantee stable growth during culture. According to the method, a large quantity of high-quality nursery stocks can be obtained within short time.

Description

technical field [0001] The invention relates to the technical field of plant vegetative propagation, in particular to a method for tissue culture and rapid propagation of unicorn tail [Epipremnumpinniatum] seedlings. technical background [0002] Kylintail, also known as Kylinleaf, Tree Dragon, and Flying Centipede, belongs to the genus Kylintail in the family Araceae, and is a perennial evergreen vine foliage plant. It is native to Guangdong, Guangxi, Hainan, Taiwan provinces and regions in southern China, as well as India, Malaysia and other countries. Its nature likes a warm, humid and shaded environment. Stems and leaves can be used for medicine, clearing heat and cooling blood, promoting blood circulation, removing blood stasis, detoxifying and reducing swelling; mainly treating cold and fever; epistaxis; red eyes and swelling pain; whooping cough; bruises; bone fractures; rheumatic arthralgia; Boil; snakebite. Kylin's caudal leaves are dark green and lustrous. The y...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 叶清梅李雪黄雨花黄惠玲简丽观钟春德涂艺辉杨双霞
Owner SUNSHINE HORTICULTURE
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