Large-scale vegetative reproduction production method of unicorn tail seedlings
A technology of asexual reproduction and production methods, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of not being able to obtain a large number of high-quality seedlings, and achieve browning in tissue culture, stable genetic traits, and uniform bud size Effect
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[0049] In the present invention, the MS improved medium used is to add 220mg.L in the conventional MS medium -1 Ca(NO 3 ) 2 .4H 2 O, and KNO 3 Dosage from 1900mg.L -1 increased to 2100mg.L -1 .
[0050] In the present invention, the peat soil used is provided by Danish Pinshi Company, and high-quality peat moss is used, and the organic matter content reaches 98%.
[0051] The large-scale asexual reproduction production method of unicorn tail seedlings of the present invention is realized by the following steps: 1, cultivation of mother plant, pretreatment and explant collection
[0052] Due to the high maturity of the branches of the adult mother plant and the high content of phenolic substances, if it is used as an explant, it will seriously affect the acquisition rate of the sterile body and prolong the induction and domestication cycle. Therefore, the present invention selects excellent, robust, no damage by diseases and insect pests, and the perennial unicorn tail w...
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