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Extraction, separation and purification method of red ginseng polysaccharide

A technology for separation and purification of ginseng polysaccharides, applied in the field of extraction and separation and purification of red ginseng polysaccharides, can solve problems such as unclear structure, low purity of red ginseng polysaccharides, unclear physical and chemical properties, etc. The effect of high protein efficiency

Active Publication Date: 2022-02-18
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a kind of polysaccharide that can realize industrial production, and solve the problems of low purity, unclear structure and unclear physical and chemical properties of the currently extracted red ginseng polysaccharides. Extraction and purification process of red ginseng polysaccharides that may be transformed into applications

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  • Extraction, separation and purification method of red ginseng polysaccharide
  • Extraction, separation and purification method of red ginseng polysaccharide
  • Extraction, separation and purification method of red ginseng polysaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0045] The method for extracting and separating and purifying red ginseng polysaccharides from red ginseng comprises the following steps:

[0046] Step (1), raw material pretreatment: according to the mass volume ratio of red ginseng decoction pieces and petroleum ether (60-90°C) is 1:4 (m / v, g:mL), add petroleum ether to red ginseng decoction pieces, let stand 12h for degreasing treatment to remove petroleum ether, dry the red ginseng decoction pieces, and smash into red ginseng particles with a size of 0.1-0.2cm;

[0047] Step (2), reflux extraction: Put the red ginseng granules into the reflux extraction equipment, add pure water according to the ratio of material to liquid (m / v, g:mL) 1:15, first soak for 2 hours, then heat and reflux extraction, set the reflux The temperature of extraction is 95°C, each extraction is 2h, extraction is 2 times, each extraction is completed and filtered, and the filtrates are combined to obtain the extract;

[0048] Step (3), alcohol precipi...

Embodiment 2

[0076] Get 1g red ginseng crude polysaccharide extract (same as embodiment 1), add 10mL purified water to dissolve, according to the mass ratio of papain (same embodiment 1) and red ginseng crude polysaccharide extract be respectively 1:3, 1:5, Add protease at 1:7, heat in water bath at 65°C for 1h, heat inactivate at 100°C for 10min, then add Sevage reagent according to polysaccharide:Sevage reagent (chloroform:n-butanol-4:1)-1:4, shake, and carry out Deproteinize, centrifuge, take the supernatant, freeze-dry and measure the absorbance at 595nm by Coomassie brilliant blue method.

[0077] It can be seen from Table 1 that when the mass ratio of papain and red ginseng crude polysaccharide extract was 1:5, the protein removal rate was 80.40%, and the polysaccharide loss rate was 18.36%. Preferably, the mass ratio of papain and red ginseng crude polysaccharide extract is 1:5.

[0078] Table 3. Effect of protease dosage on protein removal rate

[0079]

Embodiment 3

[0081] Get 1g of red ginseng crude polysaccharide extract (same as Example 1), add 10mL of purified water to dissolve, add papain according to the mass ratio of papain (same as Example 1) and red ginseng crude polysaccharide extract is 1:5, 65 ℃ Heat in a water bath for 0.5, 1, and 2 hours respectively, heat inactivation at 100°C for 10 minutes, and then follow the volume ratio of red ginseng crude polysaccharide extract solution and Sevage reagent (chloroform:n-butanol=4:1v / v) to 1:4 Add Sevage reagent, oscillate, perform deproteinization treatment, centrifuge, take supernatant, freeze-dry and measure absorbance at 595nm by Coomassie brilliant blue method.

[0082] Table 4. Effect of protease dosage on protein removal rate

[0083]

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Abstract

The invention discloses an extraction, separation and purification method of red ginseng polysaccharide. The method comprises the following steps: degreasing red ginseng decoction pieces, breaking into pieces, carrying out reflux extraction by taking water as an extraction solvent, and carrying out concentration and alcohol precipitation on an extracting solution to obtain a red ginseng crude polysaccharide extract; deproteinizing the red ginseng crude polysaccharide extract by using a protease-Sevage combined method, decolorizing supernate by using hydrogen peroxide, dialyzing, freeze-drying dialysate to obtain red ginseng crude polysaccharide; performing DEAE-52 column chromatography on the red ginseng crude polysaccharide to obtain red ginseng mixed polysaccharide, dialyzing the red ginseng mixed polysaccharide to remove salt, and finally performing SephadexG-100 gel column chromatography separation, and performing freezing and drying to obtain the red ginseng homogeneous polysaccharide RGP2-1. The extraction, separation and purification conditions are mild, and the integrity of the polysaccharide structure can be kept. The obtained three kinds of red ginseng polysaccharides are good in purity uniformity and clear in structure, and have a wide application value in the fields of medicine, health care products, food, cosmetics and the like.

Description

technical field [0001] The invention relates to a method for extracting, separating and purifying red ginseng polysaccharides. Background technique [0002] Red ginseng (Red Gingseng) is a kind of processed product after steaming the dried roots and rhizomes of ginseng (Panax ginsengC.A.Mey.). It is widely used in China, Japan, Korea and other countries with a long history. The main medicinal components of red ginseng are saponins and polysaccharides, among which the research reports on red ginseng saponins are more abundant, while the researches on the structure and function of red ginseng polysaccharides are less. Compared with ginseng polysaccharides, some activities of red ginseng polysaccharides, such as antioxidant activity, anti-fatigue activity, anti-tumor activity, immune activity, etc., are significantly improved. Therefore, red ginseng polysaccharides have broad application prospects in traditional Chinese medicine, functional food, cosmetics, etc. [0003] The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K47/36
CPCC08B37/006C08B37/0003A61K47/36
Inventor 贾晓斌廉源沛封亮王贤峰
Owner CHINA PHARM UNIV
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