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Method for researching antineoplastic effect of apatinib based on PDX model

A technology for anti-tumor effect and model research, which is applied in the field of research on the anti-tumor effect of apatinib based on PDX model, which can solve the problem of no report of tongue cancer treatment.

Pending Publication Date: 2022-02-11
南昌大学附属口腔医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Apatinib is a small-molecule anti-angiogenic targeted drug independently developed by my country. It is the first drug that has been

Method used

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  • Method for researching antineoplastic effect of apatinib based on PDX model
  • Method for researching antineoplastic effect of apatinib based on PDX model
  • Method for researching antineoplastic effect of apatinib based on PDX model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation and processing of embodiment 1 raw material

[0030] Tumor tissue samples: Tumor tissue samples came from fresh tongue cancer tumor tissues surgically resected at the Stomatological Hospital Affiliated to Nanchang University. The experimental process of the present invention was approved by the Ethics Committee of Stomatology Hospital Affiliated to Nanchang University, and all patients signed informed consent.

[0031] Experimental animals: 6-8-week-old female immunodeficient Balb / C nude mice were selected for the experiment. The mice were kept in the SPF animal room, in the IVC mouse box, and the temperature was 22-26°C. All animal experiments were approved by the Experimental Animal Welfare Ethics Committee of Nanchang Leyou Biotechnology Co., Ltd.

[0032] Experimental drugs: Apatinib (National Pharmaceutical Approval H20140103, Jiangsu Hengrui Medicine Co., Ltd.), cisplatin (National Pharmaceutical Approval H20040813, Jiangsu Hansoh Pharmaceutical ...

Embodiment 2

[0033] Example 2 Construction of Tongue Cancer PDX Model

[0034] (1) Treatment of tumor samples: Fresh tongue cancer tumor samples removed from surgery were sterilized, trimmed, rinsed and divided into 2mm×2mm×2mm micro-tissue pieces with a scalpel and placed in EP tubes containing DMEM high-glucose medium Transfer to the animal room in an ice box to prepare for inoculation.

[0035] (2) Subcutaneous inoculation: select female Balb / C nude mice, fix the mice after skin preparation on the scapula, sterilize with iodine, and inoculate the tumor tissue to the scapula with a trocar after local anesthesia with lidocaine, which is recorded as P0 generation PDX Model( figure 1 ).

[0036] (3) Subculture inoculation: when the tumor tissue grows to 1300mm 3 The tumor tissue was surgically removed, and the tissue samples were divided into 2mm×2mm×2mm tissue pieces and inoculated into the next batch of Balb / C mice to obtain the P1 generation PDX model. After 4 passages and inoculation...

Embodiment 3

[0037] Example 3 Identification of Tongue Cancer PDX Model Consistency with Patient's Tumor Tissue

[0038] (1) HE staining to detect histopathological features of PDX models

[0039] Take the tumor tissue of the patient and the PDX model, cut out a 5mm×5mm×5mm tissue block, fix it with a general-purpose tissue fixative for 24 hours, take out the tissue, and fix it in ethanol-formaldehyde fixative for 2 hours; fix it twice with 95% ethanol, 2 hours each time; 2 times of absolute ethanol fixation, 1 hour each time; 2 times of xylene fixation, 30 min each time; 3 times of paraffin fixation, 30 min, 60 min and 90 min respectively. After the tissue was fixed, it was transparentized with a clearing agent; the paraffin-embedded tissue was cut into 5 μm thick paraffin sections; the paraffin sections were dewaxed in an oven at 80°C for hydration; stained with hematoxylin, stained cell nuclei, rinsed, differentiated with 1% hydrochloric acid ethanol, and then Rinse, turn to blue after...

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Abstract

The invention relates to the technical field of drug effect detection, and provides a method for researching the antineoplastic effect of apatinib based on a PDX model, and the method comprises the following steps: (1) transplanting an in-vitro tumor tissue into a mouse body, and constructing a P0-generation PDX model; (2) carrying out passage on the P0-generation PDX model for four times to obtain a P4-generation PDX model; and (3) treating the P4-generation PDX model with an anti-tumor drug, and observing the tumor tissue inhibition effect. According to the invention, the curative effect of the antineoplastic drug apatinib on tongue cancer is researched by utilizing a PDX model, and the antineoplastic mechanism of apatinib in tongue cancer is researched.

Description

technical field [0001] The invention relates to the technical field of drug efficacy detection, in particular to a method for studying the antitumor effect of apatinib based on a PDX model. Background technique [0002] Oral cancer is the most common malignant tumor in the maxillofacial region, occurring in the tongue, cheeks, gums and other parts, mainly squamous cell carcinoma. Tongue cancer has a high incidence rate, rapid growth, high degree of malignancy, poor clinical treatment effect, and low patient survival rate. Due to the lack of good preclinical research models, the research on the pathogenesis, drug screening and treatment of tongue cancer is progressing slowly, and it is urgent to find new effective treatment methods in clinical practice. [0003] The human-derived tumor xenograft (PDX) model is a method in which tumor cells or tissues of patients are directly transplanted into mice by means of orthotopic or heterotopic (mostly subcutaneous or renal capsule), ...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K31/444A61K33/243A61P35/00A61P1/02A01K67/027
CPCA61K49/0004A61K31/444A61K33/243A61P35/00A61P1/02A01K67/0271A01K2267/0331A01K2207/12A01K2227/105A61K2300/00
Inventor 胡晓萍徐佳徐珮琼杨骏王莉贺岚沈婷艺
Owner 南昌大学附属口腔医院
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