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Preparation method and application of didymin

A technology of melissain and medicine, which is applied in the field of preparation of melissain, can solve the problems that melissain has not been found, and achieve the effects of high purity, high yield, and clear chemical structure

Pending Publication Date: 2022-02-01
GUANGXI MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the application of melissain in the treatment of non-alcoholic fatty liver disease

Method used

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  • Preparation method and application of didymin
  • Preparation method and application of didymin

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Embodiment Construction

[0020] The present invention will be described below in conjunction with the accompanying drawings, and the specific embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0021] One, the preparation of melissain

[0022] Take 10kg of Pinnaea chinensis, decoct with 10 times the volume of water for 2 hours, repeat once, add 95% ethanol after concentrating the extract and stir, let it stand overnight, filter and apply macroporous resin, use 6-10 times the column volume Washing with water, eluting with 50% ethanol, collecting the eluate, and recovering the ethanol to obtain the extract. The extract was separated by silica gel column chromatography, chloroform-methanol gradient elution, the volume ratio of chloroform and methanol was changed to 100:0-0:100, and the Sephadex LH-20 chromatography column and MCI macroporous resin were used for 3 times in sequence Separation and purification gav...

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Abstract

The invention discloses a preparation method and application of didymin. The method comprises the following steps: 1) taking a clinopodium chinense medicinal material, decocting the clinopodium chinense medicinal material with 9-11 times by volume of water for 1.5-2.5 hours, conducting decocting for 1-3 times, concentrating an extracting solution, adding 95% ethanol, conducting stirring, conducting standing overnight, conducting filtering, conducting loading on macroporous resin, washing a column with 6-10 times by volume of the column, conducting eluting with 50% ethanol, collecting an eluate, and recovering the ethanol to obtain an extract; and 2) carrying out silica gel column chromatography separation on the extract, carrying out chloroform-methanol gradient elution, and sequentially carrying out separation and purification 2-4 times by using a Sephadex LH-20 chromatographic column and MCI macroporous resin. The application refers to the application of the didymin in preparing the medicine for treating the non-alcoholic fatty liver disease. Specifically, the didymin has a protective effect on pathological injury of the liver, and can reduce formation of lipid droplets and relieve accumulation of lipid. The invention provides a novel compound with a clear structure and high efficiency for the treatment of the non-alcoholic fatty liver disease, and develops a new medication approach.

Description

technical field [0001] The invention relates to the medical use of plant extracts, in particular to a preparation method and application of melissain. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) is one of the most common chronic liver diseases in the world, and it is a fat accumulation disease caused by many factors. The pathogenesis of non-alcoholic fatty liver disease is extremely complex and is still in the hypothesis stage. At present, the second hit hypothesis is currently widely accepted theory, the first hit is caused by abnormal accumulation of lipids, and the second hit is mainly caused by inflammatory response and lipid peroxidation stress. [0003] Clinopodium chinense (Benth.) O.Kuntze is the dry aboveground part of Clinopodium chinense (Benth.) O. Kuntze, which mainly contains chemical components such as flavonoids, triterpenes and their saponins, volatile oils, phenylpropanoids, steroids, etc. Compound, melissain (Didymin) belongs ...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H17/07A61K31/7048A61P1/16
CPCC07H1/08C07H17/07A61K31/7048A61P1/16
Inventor 方斌林兴黄权芳熊钰华
Owner GUANGXI MEDICAL UNIVERSITY
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