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Method for modifying sialic acid on surface of gold nano array

A gold nanometer and sialic acid technology, which is applied in the direction of material analysis, instruments, and measuring devices through observation of the influence on chemical indicators, can solve the problems of cumbersome preparation process, long time consumption, and high colloidal gold concentration requirements, and achieve simplification The effect of the reaction step

Pending Publication Date: 2022-01-28
NANJING UNIV OF SCI & TECH
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Problems solved by technology

This method has high requirements for the concentration of colloidal gold and the concentration of target detection substances, and there are problems such as cumbersome sample preparation process, long time consumption, and strong random distribution of conjugates.

Method used

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  • Method for modifying sialic acid on surface of gold nano array
  • Method for modifying sialic acid on surface of gold nano array
  • Method for modifying sialic acid on surface of gold nano array

Examples

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Embodiment 1

[0033] Example 1: Gold Nanoarray Modification of Sialic Acid

[0034] Step S201, at room temperature, use deionized water and acetylneuraminic acid to prepare 10 mL of sialic acid solution, and then add 250 μL of HAuCl 4 After the solution is fully mixed to form a mixed solution 103, it is slowly poured into the beaker 102 containing the gold nanoarray 101 along the wall of the cup by the drainage rod 105, and the gold nanoarray is not over the gold nanoarray. The nanoarray is the sample.

[0035] In step S202, use the drainage rod 105 to slowly inject 50 μL of NaOH solution into the solution along the wall of the cup. Gently stir the upper layer of the solution to avoid affecting the gold nano-array 101, and stir the solution evenly.

[0036] Step S203, put the reaction vessel beaker 102 into the water bath heating pot 106, and heat at a constant temperature, which can be controlled by controlling the reaction time or sialic acid and HAuCl 4 The ratio of the solution is us...

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Abstract

The invention discloses a method for modifying sialic acid at the fixedly and orderly arranged gold nanodots on a gold nano array. The method is characterized in that the sialic acid is modified in situ at the gold nanodots on the surface of the gold nanoarray by using a seed crystal growth method. The sialic acid modified gold nano array is prepared by utilizing a specific binding principle of the influenza virus surface hemagglutinin and the sialic acid, so that the specific capture and recognition of the influenza viruses can be realized.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for modifying sialic acid on the surface of a gold nano array. Background technique [0002] Gold nanoparticles are a kind of nanomaterials with good biocompatibility. When the surface of gold nanoparticles is modified with small molecules, according to the principle of immunology to achieve specific coupling to pathogens, the optical properties such as absorption peaks and scattering will change. , researchers have conducted related research on the changes in the scattering patterns of gold nanoparticles before and after coupling to viruses, so as to achieve specific detection of viruses. At present, colloidal gold modified with antibodies is mainly used to specifically couple biological macromolecules such as viruses, and qualitative or quantitative analysis of target detection substances is carried out through macroscopic color changes. This method has high requirements ...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/786
Inventor 熊吉川徐厚祥刘学峰张衡金霄徐彬刘卫平倪斌
Owner NANJING UNIV OF SCI & TECH
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