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Method for bioelectrocatalytic reduction of carbon dioxide by mesoporous material immobilized enzyme and application

A technology of mesoporous materials and immobilized enzymes, applied in biochemical equipment and methods, immobilized enzymes, oxidoreductases, etc., can solve the problems of expensive coenzyme NADH, low solubility, unfavorable reaction conversion, easy inactivation, etc., to achieve Effect of promoting CO2 reduction reaction, reducing coenzyme consumption, and maintaining enzyme activity

Pending Publication Date: 2022-01-28
HEBEI UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, free formate dehydrogenase has problems such as easy inactivation, difficult recovery, and high cost when catalyzing the reaction. 2 Low solubility in solution is not conducive to reaction conversion, and the coenzyme NADH consumed by the reaction is expensive

Method used

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  • Method for bioelectrocatalytic reduction of carbon dioxide by mesoporous material immobilized enzyme and application
  • Method for bioelectrocatalytic reduction of carbon dioxide by mesoporous material immobilized enzyme and application
  • Method for bioelectrocatalytic reduction of carbon dioxide by mesoporous material immobilized enzyme and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] bioelectrocatalytic CO 2 To restore, the steps are as follows:

[0026] Step 1) Weigh 1 g of commercial mesoporous material SBA-15 into a 100 mL single-necked flask, add 30 mL of anhydrous toluene, and ultrasonically disperse to form a homogeneous solution. Then, slowly add 1 mL of silane coupling agent 3-aminopropyltriethoxysilane (APTES) into the homogeneous solution, stir at 110° C., condense and reflux for 24 hours. The reaction solution was cooled to room temperature, centrifuged, washed three times with absolute ethanol to remove the unreacted silane coupling agent, and then dried in an oven at 60°C overnight to obtain an APTES-modified mesoporous material.

[0027] Step 2) Weigh 0.3 g of the APTES-modified mesoporous material prepared in step 1) and place it in a 100 mL one-necked flask, add 10 mL of phosphate buffer, and disperse by ultrasonication. Add 1 mL of glutaraldehyde solution to the above solution and stir, then add 30 mL of 100 mM phosphate buffer co...

Embodiment 2

[0035] bioelectrocatalytic CO 2 Reduction, the specific steps are basically the same as in Example 1, except that in step b), 100 mM phosphate buffer containing 0.09 g PEI is added, so that the mass ratio of PEI to SBA-15 is 30%.

[0036] Figure 4 is the CO of SBA-15 under different amounts of PEI modification 2 Temperature-programmed desorption characterization diagram, it can be seen that after PEI modification, the mesoporous material has a 2 The adsorption capacity increased significantly.

[0037] Using Example 2 for bioelectrocatalytic CO 2 Reduction, the concentration of formic acid produced after 180 minutes of testing is 0.88mM.

Embodiment 3

[0039] bioelectrocatalytic CO 2 Reduction, the specific steps are basically the same as in Example 1, except that 100 mM phosphate buffer containing 0.24 g PEI is added in step b), so that the mass ratio of PEI to SBA-15 is 80%.

[0040] Using Example 3 for bioelectrocatalytic CO 2 Reduction, the concentration of formic acid produced after testing for 180min is 1.10mM.

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Abstract

The invention discloses a method for bioelectrocatalytic reduction of carbon dioxide by a mesoporous material immobilized enzyme, which comprises the following steps of: modifying SBA-15 serving as the mesoporous material through 3-aminopropyltriethoxysilane (APTES), covalently combining glutaraldehyde with polyethyleneimine (PEI) to realize amination of the mesoporous material, and oscillating and mixing to immobilize formate dehydrogenase, adopting neutral red coenzyme for regeneration, and realizing bioelectrocatalysis CO2 reduction on the cathode. The preparation method is simple and convenient to operate and convenient to implement, formate dehydrogenase is immobilized on the mesoporous material through PEI, the adsorption capacity of substrate CO2 can be improved through PEI, consumption of expensive coenzyme can be reduced through regeneration of neutral red coenzyme, and high formic acid yield is achieved through bioelectrocatalysis CO2 reduction.

Description

technical field [0001] The invention relates to a kind of mesoporous material immobilized enzyme for bioelectrocatalytic CO 2 The reduction method and application specifically relate to the preparation of a high-efficiency mesoporous carrier based on glutaraldehyde cross-linked PEI, the immobilized enzyme and the application of bioelectrocatalysis. Background technique [0002] The development of modern industry has led to an increasing demand for carbon-containing fossil fuels, and the CO released by their combustion 2 leading to the intensification of the greenhouse effect. Improve the climate and reduce CO 2 Emissions have become one of the hotspots of modern research. In recent years, CO 2 Capture, storage, reduction and conversion technologies continue to evolve, especially with the use of CO 2 The production of energy and fuel compounds such as CO, methane, methanol, formaldehyde and formic acid can not only meet the growing demand for chemical industry and energy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C25B3/26C25B11/054C25B11/095C12N9/02C12N11/089C12N11/14C12P7/40C25B1/23C25B3/03C25B3/07
CPCC25B3/26C25B3/03C25B3/07C25B1/23C25B11/095C25B11/054C12N11/14C12N11/089C12N9/0008C12P7/40C12Y102/01002
Inventor 刘冠华陈冉陈华勋杨明辉王泽惠周丽亚姜艳军高静
Owner HEBEI UNIV OF TECH
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