Method for determining kinetic parameters of lysophosphatidic acid acyltransferase
A technology of lysophosphatidic acid and acyl transfer, applied in acyltransferase, transferase, chemical instruments and methods, etc., can solve the problems of high substrate and human disadvantage, and achieve harm avoidance, high-sensitivity detection ability, and high-efficiency separation ability. Effect
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[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0021] see Figure 1-2 , an embodiment provided by the present invention: the method for measuring the kinetic parameters of lysophosphatidic acid acyltransferase comprises the following steps: Step 1, leaf transient expression and vector construction; Step 2, Agrobacterium-mediated transient expression in Ben's leaves Agrobacterium strain; step 3, extraction of leaf cell microsomes; step 4, in vitro LPAAT reaction; step 5, LC-MS lipidomics determination;
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