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Composition for treating or preventing eye diseases and application thereof

A technology for eye diseases and compositions, applied in the field of medicine, can solve problems affecting eye health, the number and volume of macular nerves become smaller, unstable vision, etc., and achieve prevention and improvement of retinal function, a wide range of safe doses, and treatment Or the effect of preventing eye diseases

Pending Publication Date: 2022-01-21
GUANGZHOU WANGLAOJI MAJOR HEALTH IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of ganglion cells are gathered here, and the deposition of Aβ protein will damage these nerve cells, resulting in a decrease in the number and volume of macular nerves, atrophy of nerve fibers, damage to the function of the optical center, darkening, deformation, and loss of vision when seeing things. resulting in a loss of visual quality
These age-related eye degenerative diseases, unhygienic use of eyes, or use of unsafe eye products may affect eye health, resulting in blurred vision and unstable vision. Eye problems such as eye swelling, headache, photophobia, sensitivity to light, and difficulty focusing when switching from near vision to distance vision

Method used

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  • Composition for treating or preventing eye diseases and application thereof
  • Composition for treating or preventing eye diseases and application thereof
  • Composition for treating or preventing eye diseases and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1. the preparation of Wanglaoji extract of the present invention

[0032] The Wanglaoji herbal tea extract (WHT) in the following examples is composed of the following active ingredients: 2 parts of jelly grass, 0.5 part of frangipani, 0.5 part of cloth dregs, 1 part of chrysanthemum, 2 parts of honeysuckle, 0.5 part of Prunella vulgaris, 0.5 part of licorice . 30-70% ethanol is slightly boiled and extracted twice, each time for 2 hours, and the supernatant is collected and concentrated with water to obtain a herbal tea extract with a solid content of about 55±3%. 1g of herbal tea extract is equivalent to 8.47g of crude drugs, and the total flavonoid content is 1.2×10 2 mg / 100ml, the extract has a special smell, slightly sweet herbal tea extract. In some embodiments, the extract can be extracted with water. The following dosages are expressed in extract dosage.

[0033] Statistical Analysis

Embodiment 2

[0035] Example 2. Effect of WHT on primary microglial cells under Aβ stimulation

[0036] Isolation and culture of primary retinal microglial cells: C57BL / 6J mice were obtained from the Animal Center of the University of Hong Kong (Jackson Lab strain, USA). Take a newborn mouse (1-4 days after birth), take the eyeball and peel off the retina, carefully separate and remove the choroidal blood vessels, cut it into pieces and transfer it to a centrifuge tube, add 3% papain, put it in a water bath at 37°C for 20 minutes, and then digest it for 4 minutes. Centrifuge at 1,000 rpm for 5 min. Discard the supernatant, add a certain amount of DMEM / F12 (10% fetal bovine serum) medium to resuspend the cells, inoculate them into a culture dish, change the medium after 24 hours, and then change the medium every 3 days, 10-14 Three days later, trypsinization was performed, and the culture was shaken at 45 rpm for 20-25 minutes, and the cells in the upper layer were isolated as retinal micro...

Embodiment 3

[0042] Example 3. In vivo efficacy study: 5xFAD transgenic mice and WHT gavage test

[0043] The Alzheimer's disease model mice used in this experiment are transgenic AD mice with 5xFAD mutation (B6.Cg-TgAPPSwFlLon, PSEN1*M146L*L286V, 6799Vas / Mmjax) [6] Purchased from Jackson Laboratory (MMRRC stock#34848), without retinal degeneration gene Pde6b rd1. 5xFAD transgenic mice overexpress mutated human APP (695) and Swedish-type (K670N, M671L), Florida-type (I716V) and London-type (V717I) familial Alzheimer's disease (FAD) mutations and carry two FAD Human PS1 with mutations M146L and L286V is transcriptionally controlled by the neuron-specific mouse Thy1 promoter. C57BL / 6J female mice were used to cross with 5xFAD male mice, and the F1 generation was produced for genotype identification. Mice with the mutant gene were used as experimental mice, and wild-type littermates without the mutant gene were used as controls mice. The experimental animals are all kept in a brown transp...

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PUM

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Abstract

The invention relates to a composition for treating or preventing eye diseases and application thereof, the composition is in an extract form, and the total flavonoid content of the extract is greater than or equal to 120mg / 100ml; the composition comprises the following components: mesona chinensis, plumeria rubra, microcos paniculata, chrysanthemum, honeysuckle, selfheal and liquorice. The composition disclosed by the invention is wide in safe dosage range, has a protective effect on retinal nerve cells, can prevent and improve the retina function, and has an effect of treating or preventing eye diseases.

Description

technical field [0001] The invention belongs to the field of medicine, in particular to a composition for treating or preventing eye diseases and its application Background technique [0002] Eyes are the most important organ in human senses, and about 80% of the knowledge and memory in the brain are directly obtained through the eyes. Eye health is not only an important indicator of physical health, but also related to a person's lifelong growth and development. Glaucoma is the second leading cause of blindness in the world, and it is irreversible blindness. In addition to being related to intraocular pressure, glaucoma is also related to systemic degenerative diseases. Whenever we are tired of life and study, we will choose to rest on the desk for a while, but in the process of falling asleep, once the eyes are pressed on the arms for too long, it will cause pressure on the eyes, which lasts for a long time, and it is easy to cause corneal damage. and retinal damage, le...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/536A23L33/105A61P27/02A61P25/00A61P9/10
CPCA61K36/53A61K36/24A61K36/287A61K36/355A61K36/536A61K36/484A61K9/0053A61K9/0048A61P27/02A61P9/10A61P25/00A23L33/105A23V2002/00A61K2300/00A23V2200/30A23V2250/21
Inventor 翁少全苏国辉徐文流李词周赵健郑荣波张丰源王宇
Owner GUANGZHOU WANGLAOJI MAJOR HEALTH IND
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