Preparation method and application of proteophilic and plant-philic nitrogen source probiotic solid culture medium

A technology of solid culture medium and probiotics, which is applied in the field of solid culture medium of probiotics probiotics that are proteotropic and phytophilic nitrogen sources, can solve the problem that fermentation propagation technology has not been widely popularized, the popularization of self-cultivation at home has not been realized, and the market price of bacteria can be solved. Expensive and other problems, to achieve the effect of reducing application cost, reducing difficulty and cost, and low circulation cost

Inactive Publication Date: 2022-01-21
优仕康生(天津)科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cultivation of the human body's own probiotics, especially proteinophilic anaerobic probiotics, which have significant beneficial effects on the human body has relatively high requirements, and the cultivation of the strains still needs to be carried out in laboratories and biological preparation production institutions, and the fermentation and reproduction technology has not yet been developed. Widely popularized, especially failed to achieve the popularization of self-cultivation at home
In addition, due to the high commercial price of this type of bacteria, its universal application in the field of health care is also limited.

Method used

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  • Preparation method and application of proteophilic and plant-philic nitrogen source probiotic solid culture medium
  • Preparation method and application of proteophilic and plant-philic nitrogen source probiotic solid culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The preparation method of the present embodiment is the following steps:

[0054] (1) Ingredients The proteinophilic and phytophilic nitrogen source probiotic solid medium is composed of nougatose basic ingredients, animal non-dairy non-broth protein nitrogen source and edible fungal nitrogen source, and prebiotic oligosaccharides in fractional ratio; Among them, 60-70% of the basic ingredients of nougat by weight, 10-15% of animal non-dairy and non-broth protein source (cooked dry powder), 10-15% of edible fungal nitrogen source (dry powder), and probiotics Meta oligosaccharides 10-15%;

[0055] (2) Preparation (1) Making sugar with nougat ingredients and preparation methods (2) Adding animal non-dairy and non-broth protein according to the weight to weight ratio when the sugar embryo is cooled to 80-100°C and is in a fluid or semi-fluid state Source (cooked dry powder) and edible fungal nitrogen source (dry powder) and stir or knead to make the added ingredients even...

Embodiment 2

[0068] The present embodiment is the same as Example 1, except that the bird's nest and donkey-hide gelatin are replaced by an equivalent amount of beef or pork floss; the agaricus blazei is used to replace matsutake, and the beef floss is commercially available beef or pork velvet (cooked lean meat fine short fiber) )loose

Embodiment 3

[0070] The preparation method of the present embodiment is the following steps:

[0071] (1) Ingredients malt milk extract (commercially available product), animal non-dairy non-broth protein source and edible fungal nitrogen source are composed according to fractional ratio; among them, malt milk extract is 60-70% by weight and animal non-dairy non-broth protein source Broth protein source (cooked dry powder) 10-15%, edible fungal nitrogen source (dry powder) 10-15%; prebiotic oligosaccharides 10-15%;

[0072](2) Preparation Accurately weigh the four types of powder ingredients in proportion and mix them uniformly, and make a probiotic powder solid culture medium after mixing, and the water content is less than 5%;

[0073] (3) Packaging and storage The powdered solid culture medium prepared in Preparation 2 is subpackaged, packed into the required gram weight, and stored at normal temperature or under dry conditions.

[0074] The concrete batching formula of this embodiment...

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Abstract

The invention relates to a preparation method and application of a proteophilic and plant-philic nitrogen source probiotic solid culture medium. The solid culture medium is prepared from traditional nougat or wheat milk essence as a basic ingredient, an animal non-milk non-broth protein source, an edible fungus plant nitrogen source and prebiotic oligosaccharide according to a mass-gram weight ratio. The basic ingredients of the nougat or the wheat milk essence account for 60-70%, the animal non-milk non-broth protein source (cooked dry powder) accounts for 10-15%, the edible fungus plant nitrogen source (dry powder) accounts for 10-15%, and the prebiotic oligosaccharide accounts for 10-15%; and the animal non-milk non-broth protein source, the fungus plant fiber nitrogen source and the prebiotic oligosaccharide are added in the preparation process of the wheat milk essence or the nougat to form a small package with one dosage, and the small package is opened and brewed once to obtain a probiotic convenient culture medium and a high-nutrition functional beverage with a good taste. Cuttlefish ink, donkey-hide gelatin, cubilose, tricholoma matsutake and other unconventional edible dry powder are used as main base materials, and the innovation of the solid culture medium is shown.

Description

technical field [0001] The invention belongs to the fields of food, beverage and biotechnology, and in particular relates to a preparation method and application of a solid culture medium for proteinophilic and phytophilic nitrogen source probiotics. Background technique [0002] The cultivation and utilization of probiotics is an important field of biotechnology in medicine and functional drinks. In recent decades, the cultivation and utilization of probiotics has been widely popularized in China. However, the cultivation of the human body's own probiotics, especially proteinophilic anaerobic probiotics, which have significant beneficial effects on the human body has relatively high requirements, and the cultivation of the strains still needs to be carried out in laboratories and biological preparation production institutions, and the fermentation and reproduction technology has not yet been developed. Widely popularized, especially failed to realize the popularization of s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/39A23L2/52A23L33/135A23L33/17A23L33/21A23L33/10A23L2/60C12N1/20C12N1/16C12R1/01C12R1/645
CPCA23L2/39A23L2/52A23L33/135A23L33/17A23L33/21A23L33/10A23L2/60C12N1/20C12N1/16A23V2400/51
Inventor 刘金良刘萍
Owner 优仕康生(天津)科技发展有限公司
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