Method for prolonging storage time of Aenasius bambawalei Hayat and application thereof

A technology of Bancroft's jumping bee and storage time, which is applied in the field of low-temperature storage of insects, can solve the problems of prolonging the life of natural enemies, etc., and achieve the effect of improving low-temperature storage time, improving cold resistance, and prolonging the life of female bees

Active Publication Date: 2022-01-21
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the actual application process, this storage period is far from meeting the needs of prolonging the life of natural enemies and providing sufficient and stable natural enemy products for biological control

Method used

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  • Method for prolonging storage time of Aenasius bambawalei Hayat and application thereof
  • Method for prolonging storage time of Aenasius bambawalei Hayat and application thereof
  • Method for prolonging storage time of Aenasius bambawalei Hayat and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 Effect of different ages of low-temperature treatment on Leaperia bancroft on its eclosion rate

[0026] The total developmental period of Le. bancrofti at room temperature is 14.5 days, of which the egg, 1st instar larvae, 2nd instar larvae, 3rd instar larvae, 4th instar larvae, prepupa and pupae are 1.5d, 1d, 1d respectively , 2d, 2.5d, 1d and 5.5d. Therefore, the female adults of Pythococcus hibiscus were selected 1d, 2d, 3d, 5d, 8d, 9d and 12d after being parasitized by E. bancrofti, and they were connected to germinated potatoes, and then the potatoes with worms were placed in medical The storage test was carried out in a refrigerator, and the storage condition was 10 days at 4°C. There were 7 treatments in total, and 10 replicates for each treatment. The B. bancrofti that had not been stored in low temperature was used as the control of each treatment. After the end of the storage period, take out the fuso cotton mealybug (Pythias sinensis) and plac...

Embodiment 2

[0031] Embodiment 2 Effects of Low Temperature Acclimatization on the Eclosion of Leaperia bancroft Adults

[0032] Five different temperatures (12°C, 14°C, 16°C, 18°C ​​and 20°C) and five different photoperiods (L8:D16, L10:D14, L12:D12, L14:D10 and L16:D8) were selected for the experiment , a total of 25 treatments were set up, and 30 female adults of Pythococcus hibiscus were placed in each treatment for 7 days after being parasitized by A. bancrofti. Then, put the above parasitized Pythococcus hibiscus into the light incubator with corresponding conditions for treatment, and observe and record the survival rate of Pythococcus hibiscus and the emergence of P. bancrofti every day until there is no Jump until the bee emerges. In the present invention, the judging method until the eclosion of B. bancrofti is as follows: after the parasitic wasp is parasitized, observe whether there are eclosion holes on the scorpion scales day by day, if no eclosion holes have been seen after...

Embodiment 3

[0040] Embodiment 3 Effects of low temperature and short light treatment at different times on the emergence of Leaperia bancroft

[0041] Collect the female adults of Pythococcus hibiscus 7 days after being parasitized by E. bancrofti, place them in an environment with a temperature of 14°C and a photoperiod of L8:D16, treat them for 2 days, 4 days, 6 days, 8 days and 10 days respectively, and then transfer them to room temperature Continue feeding in the environment, wherein the conditions of the normal temperature environment are room temperature 26-28° C., relative humidity 70%-80%, and photoperiod L16:D8. The experiment consisted of 5 treatments, each treatment was repeated 3 times, and 30 parasitized female adults of P. fuso were placed in each repetition. The parasitoids reared at normal temperature were used as the control.

[0042] Table 4 Effects of low temperature and short light treatment at different times on the emergence of Leapertia bancroft

[0043]

[00...

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Abstract

The invention provides a method for prolonging the storage time of Aenasius bambawalei Hayat and application thereof, and belongs to the technical field of low-temperature storage of insects. According to the method for prolonging the storage time of the Aenasius bambawalei Hayat, provided by the invention, the Aenasius bambawalei Hayat is subjected to low-temperature domestication and then is subjected to cold storage. According to the method disclosed by the invention, low-temperature domestication is adopted to pre-treat the Aenasius bambawalei Hayat, so that the Aenasius bambawalei Hayat is in a development stagnation state, the cold resistance of the Aenasius bambawalei Hayat is improved, the low-temperature storage time of the Aenasius bambawalei Hayat is remarkably prolonged, and a technical support is provided for improving the application potential of the Aenasius bambawalei Hayat. Results of the embodiment show that according to the method provided by the invention, the refrigeration time of the Aenasius bambawalei Hayat can be prolonged to 80 days, the survival rate and reproductive capacity of the Aenasius bambawalei Hayat are not influenced, and the life of female bees of the Aenasius bambawalei Hayat can be remarkably prolonged.

Description

technical field [0001] The invention belongs to the technical field of low-temperature storage of insects, and in particular relates to a method and an application for prolonging the storage time of Leucopteris bancrofti. Background technique [0002] Both Phenacoccus soleopsis Tinsley and Phenacoccus solani Ferris belong to the family Phenacoccus of Hemiptera, and they are newly recorded invasive pests in mainland China in recent years. , traditional Chinese medicine, etc. cause serious harm. Aenasius bambawalei Hayat (Aenasius bambawalei Hayat), belonging to the Hymenoptera family Aenasius bambawalei, is the dominant obligate parasitoid of Fuso mealybug. In addition, after a short-term adaptation, the parasitic wasp can successfully parasitize another Amaryllidin spp. which has not been reported as a natural enemy. Therefore, the bee has a good application prospect of biological control. [0003] When using natural enemy insects to control agricultural and forestry dise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A01N1/02
CPCA01K67/033A01N1/0284
Inventor 张娟黄俊唐娅媛李明江
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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