Metabonomics analysis method of multi-dimensional MXene nano material

Abstract

The invention relates to the field of nano material preparation and metabonomics analysis, in particular to a metabonomics analysis method of a multi-dimensional MXene nano material. The metabonomics analysis method provided by the invention comprises the following steps: (1) carrying out DESI-MSI scanning on a biological sample to obtain DESI-MSI detection results of water-soluble metabolites and fat-soluble metabolites; the biological sample being collected from an animal model given with a multi-dimensional MXene nanomaterial; wherein the DESI-MSI detection conditions comprise that in a positive mode, the voltage is 5-6 kV; in the negative mode, the voltage being -4.5 to -5 kV; the spatial resolution being 50 to 80 [mu] m; the solvent spray being a 90-95% v / v methanol aqueous solution containing 0.1-0.5% v / v formic acid; and (2) carrying out space metabonomics analysis on the DESI-MSI detection result. In addition, the metabonomics analysis method disclosed by the invention can also comprise the step of carrying out non-targeted metabonomics detection and analysis on the cells co-cultured by the multi-dimensional MXene nano material. The method provides a basis for evaluating a multi-dimensional MXene nano material synthesis technology and biological response characteristic evaluation, and has a wide application prospect.

Description

technical field [0001] The invention relates to the field of metabolomics analysis, in particular to a metabolomics analysis method for multidimensional MXene nanomaterials. Background technique [0002] Ti 3 C 2 (MXene) nanomaterials have excellent physical and chemical properties and biochemical activities. In particular, multi-dimensional MXene nanomaterials, including zero-dimensional (quantum dot-like), two-dimensional (planar or sheet-like) MXene materials, have become the subject of various researches because of their high electrical conductivity, easy surface modification, and good mechanical properties. A promising new star in the field. Recently, multidimensional MXenes have seen increasing applications in multiple biomedical fields such as biomimicry, organ regeneration, bioimaging, antibacterial, and photothermal therapy. In order to better apply multidimensional MXenes in the biomedical field, it is usually necessary to study the intrinsic intervention proce...

AI Technical Summary

Problems solved by technology

The main reason is that people still have little understanding of the interaction between multidimensional MXene nanomaterials and organisms, and the internal intervention process and molecular mechanism of the interaction system are still unclear, so it is impossible to determine the metabolome based on the target metabolites to be detected. Processing conditions, extraction conditions and detection conditions of biological samples in chemical analysis methods

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