Kit for rapidly detecting hantavirus SEOV-S4 subtype and detection method thereof

Abstract

The invention discloses a kit for rapidly detecting hantavirus SEOV-S4 subtype and a detection method thereof. The method comprises a specific isothermal amplification primer and a corresponding rProbe probe, wherein the specific isothermal amplification primer is designed according to a conservative S fragment nucleic acid sequence of the hantavirus SEOV-S4 subtype, and the rProbe probe can be digested by RNase H and contains RNA basic groups; and a fluorophore and a quenching group are respectively designed at two ends of the rProbe, RNA basic groups are cut through RNase to be free, the hantavirus SEOV-S4 subtype can be rapidly detected by utilizing the activity of DNA polymerase and efficient isothermal amplification primers and probes, and the method has the advantages of high sensitivity, stability and specificity and very high practical application value.

Description

technical field [0001] The invention relates to a kit for rapidly detecting Hantavirus SEOV-S4 subtype and a detection method thereof, belonging to the technical field of molecular biology. Background technique [0002] Hantavirus belongs to the genus Hantavirus in the Bunyaviridae family and can cause two severe acute infectious diseases clinically, namely hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. China is the country most affected by hantavirus, with a wide distribution of cases, and the number of cases and deaths ranks first in the world. Its genome is segmented single-stranded negative-strand RNA, which consists of three RNA segments, S, M, and L, and each gene segment has only one open reading frame. [0003] Hantavirus was first discovered in North Korea in the 1950s, when more than 3,000 United Nations soldiers contracted "Korean hemorrhagic fever," or hemorrhagic fever with renal syndrome. The second outbreak of the disease in the Fou...

AI Technical Summary

Problems solved by technology

[0004] Polymerase chain reaction is currently the most widely used nucleic acid amplification technology. However, in practice, PCR technology requires the use of PCR instruments, electrophoresis instruments and other instruments, and the temperature needs to be adjusted up and down during the experiment. It also makes the technology highly dependent on the PCR machine, and at the same time it takes a long time to complete an experiment, which is not convenient and fast enough, and the cost is high. It is not suitable for large-scale rapid detection on the spot and some areas with insufficient conditions. An ideal method, which is an insurmountable limitation of ordinary PCR technology at present. Due to the serious harm of hantavirus in our country, some genotypes or subtypes of hantavirus lack rapid diagnostic detection methods. It also leads to the inability to quickly determine the subtype of population infection and trace the source of pathogens, and it is urgent to establish specific detection methods for related subtypes

Images