A kind of synthesis method of dominant malachite green hapten and its application of hapten

A malachite green, synthetic method technology, applied in peptide preparation methods, chemical instruments and methods, cyanide reaction preparation, etc., can solve the problems of weak antibody specificity and sensitivity, and achieve high specificity and sensitivity, operation method Simple and fast, high sensitivity effect

Active Publication Date: 2022-03-08
信达安检测技术(天津)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Among the existing products, the antibody specificity and sensitivity are weak, which cannot meet the existing needs

Method used

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  • A kind of synthesis method of dominant malachite green hapten and its application of hapten
  • A kind of synthesis method of dominant malachite green hapten and its application of hapten
  • A kind of synthesis method of dominant malachite green hapten and its application of hapten

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Dissolve 4-formylphenylboronic acid, acrylic acid and dimerized hydroxyl (1,5-cyclooctadiene) rhodium (I) at a molar ratio of 1:0.4:0.01 in 50ml degassed pure water, and replace the system with Ar protection , raised the temperature to 75°C and stirred for 16 hours, then lowered to room temperature, extracted the reaction solution 3 times with 60ml ethyl acetate, combined the organic phases, dried over anhydrous sodium sulfate, concentrated in vacuo, and applied the residue to silica gel column chromatography (CH 2 Cl 2 / EtOAc=9 / 1) to obtain compound A, namely 3-(4-formyl-phenyl)-propionic acid.

[0045] Put compound A, zinc chloride, and dimethylaniline with a molar ratio of 1:3:3 in 50ml of absolute ethanol, replace the system with Ar protection, heat to 90°C and stir for 24 hours. After the reaction is complete, concentrate the reaction solution , the residue was added with 25ml of methanol, acidified with 1M hydrochloric acid to pH = 3-4, and filtered to obtain lig...

Embodiment 2

[0048] The difference from Example 1 is that the molar ratio of 4-formylphenylboronic acid, acrylic acid and dimerized hydroxyl (1,5-cyclooctadiene) rhodium (I) in this example is 1:1:0.01.

[0049] Different from Example 1, the molar ratio of compound A, zinc chloride and dimethylaniline in this example is 1:3.5:3.

Embodiment 3

[0051] The difference from Example 1 is that the molar ratio of 4-formylphenylboronic acid, acrylic acid and dimerized hydroxyl (1,5-cyclooctadiene) rhodium (I) in this example is 1:0.6:0.01.

[0052] Different from Example 1, the molar ratio of compound A, zinc chloride and dimethylaniline in this example is 1:3.5:3.5.

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Abstract

The invention provides a method for synthesizing a dominant malachite green hapten and the application of the hapten, comprising the following steps: coupling 4-formylphenylboronic acid and acrylic acid under rhodium catalysis to obtain 3-(4-formyl- phenyl)-propionic acid, which reacts with N,N-dimethylaniline in the presence of zinc chloride to give the recessive malachite green hapten, which is then oxidized by tetrachloro-p-benzoquinone to give the dominant malachite green hapten Antigen, the hapten is coupled with carrier protein to obtain dominant malachite green artificial antigen. The experimental results show that the antibody produced by the antigen immunized animal of the present invention has high specificity and high sensitivity, and the obtained antiserum titer can reach 10 6 , the detection limit is 0.1ug / L.

Description

technical field [0001] The invention relates to a method for synthesizing a dominant malachite green hapten and the application of the hapten, belonging to the technical field of biochemical industry. Background technique [0002] Malachite green, also known as alkaline green, malachite green and Chinese green, is a green crystal with metallic luster. The chemical name is tetramethyldiaminotriphenylmethane. It is easily soluble in water, methanol and ethanol, and the solution is blue-green. . Malachite green is a kind of artificially synthesized triphenylmethane industrial fuel, which is mainly used as a color agent in pottery, textile, leather and food industries and a staining agent in cell chemistry. Malachite green has bactericidal and insecticidal effects, and is widely used as an insect repellant and insecticide to kill parasites in aquatic animals, protozoa and molds in fish eggs. [0003] CN103012193A discloses a malachite green hapten, an artificial antigen and a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C249/02C07C251/30C07C227/10C07C229/42C07C51/353C07C59/74C07K14/765C07K14/795C07K14/77C07K1/107C07K16/44G01N33/577G01N33/53
CPCC07C249/02C07C227/10C07C51/353C07K14/765C07K14/795C07K14/77C07K16/44G01N33/577G01N33/5308C07C59/74C07C229/42C07C251/30
Inventor 王永旭朱金端郭志远郭志雄葛怀娜于兴华
Owner 信达安检测技术(天津)有限公司
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