Stem cell three-dimensional odontogenic induction differentiation method and application

Abstract

The invention provides a stem cell three-dimensional odontogenic induction differentiation differentiation method and an application. The method is implemented by the following technical scheme: performing decalcification treatment on human or animal dentin matrixes to open dentin tubules of the human or animal dentin matrixes so as to facilitate release of odontogenic induction factors, putting the dentin matrixes into a low-adsorption culture dish, inoculating a proper amount of stem cells, performing inoculating with an appropriate amount of stem cells, and after culturing for a certain time, inducing cell adhesion and differentiation. A cell three-dimensional induction differentiation culture material comprises dentin matrixes of different species sources, such as human, pig, cattle and the like, and also comprises dentin matrixes with different decalcification degrees. The low-adsorption culture dish is not limited to culture dishes produced by specific companies and other technologies for preventing stem cells from adhering to the dish bottom are also included. The stem cells are not limited to odontogenic stem cells, and also include embryonic stem cells, umbilical cord stem cells, induced pluripotent stem cells, mesenchymal stem cells and the like.

Description

technical field [0001] The invention relates to the field of dental regeneration, in particular to a method and application for inducing three-dimensional odontogenic differentiation of stem cells. Background technique [0002] Tooth loss caused by periodontitis and other diseases and trauma is one of the common and frequently-occurring diseases of human beings, which seriously affects the patient's mastication, speech, aesthetics and mental health. The current treatment methods for missing teeth include removable dentures, fixed dentures and dental implants. These three methods are non-biological repair methods, which have many disadvantages, such as limited recovery of physiological functions, short service life and other defects, and the inability to achieve true physiological functional repair. The use of stem cells and tissue engineering technology to achieve tooth regeneration has become a hotspot in the current international research of stomatology, providing a new s...

AI Technical Summary

Problems solved by technology

[0004] At present, the main induction culture method of stem cells is mostly two-dimensional culture, which only supports the growth and differentiation of stem cells on one plane, and cannot reproduce the real three-dimensional microenvironment of cells in vivo

The two-dimensional culture environment is far inferior to the three-dimensional culture in many aspects such as biological activity and nutrient release, so that the stem cells gradually lose their original traits, shape, structure and function, resulting in often inconsistent research results and in vivo test results. The culture environment provided is very different from the microenvironment in vivo, which will inevitably have a negative effect on the proliferation and differentiation of stem cells

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