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Dynamic cell culture device and single-species cell culture device

A technology of cell culture and dynamic culture, which is applied in the direction of tissue cell/virus culture devices, biochemical instruments, biochemical equipment and methods, etc. It can solve the problems that evaluation is difficult to carry out effectively, the culture environment is unstable, and the culture conditions are difficult to control in real time.

Pending Publication Date: 2021-12-21
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the organoid culture that simulates human organs, there are mostly 1 or 2 types of cells, the simultaneous cultivation of multiple types of cells is difficult, the culture environment is unstable, and the culture conditions are difficult to control in real time, which will also make it difficult to effectively carry out later evaluation.

Method used

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  • Dynamic cell culture device and single-species cell culture device
  • Dynamic cell culture device and single-species cell culture device
  • Dynamic cell culture device and single-species cell culture device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The cell dynamic culture device of this embodiment, the structural schematic diagram is as follows Figure 1 ~ Figure 4 As shown, it includes a cell culture module, a culture medium supply module, a culture gas supply module, a culture temperature adjustment control system, and an oscillation culture adjustment control system.

[0040] (1) Cell culture module

[0041] The cell culture module includes a single cell culture vessel such as figure 1 As shown, a schematic diagram of a single cell culture vessel used independently is shown.

[0042]The single-type cell culture device includes a culture device body 1 , and the culture device body 1 is formed into a flat hollow cylinder by docking a first half cylinder 100 and a second half cylinder 200 . The first semi-cylindrical body 100 comprises a first semi-cylindrical body 101, a first base plate 102 and a first cover plate 103, and the three surround a semi-cylindrical cavity with one side opening; the first semi-cyli...

Embodiment 2

[0066] The cell dynamic culture device of the present embodiment, such as Figure 5 As shown, compared with Example 1, the main reason is that the number and connection mode of a single cell culture device in the cell culture module are different.

[0067] Figure 5 It shows that 4 single-type cell culture devices are connected in series to form a variety of cell culture device arrays. The array is not limited to 4 single-type cell culture devices, and more than or equal to 2 cell culture devices can be connected in series in this way to form a series array.

[0068] The flow of culture gas and medium is as follows Figure 5 shown. This model can simulate the environment of human tissues and organs that have requirements for the flow sequence of culture medium and culture gas, such as studying the influence of PM2.5 pollutants on the epidermal cells of the respiratory system. The components of the medium are added to the medium, and the components of the PM2.5 pollutants th...

Embodiment 3

[0071] The cell dynamic culture device of the present embodiment, such as Figure 6 As shown, compared with Example 1, the main reason is that the number and connection mode of a single cell culture device in the cell culture module are different.

[0072] The figure shows that 4 single cell culture devices are connected in parallel to form a variety of cell culture device arrays. The array is not limited to 4 single cell culture devices, and more than or equal to 2 cell culture devices can be connected in parallel to form a parallel array. .

[0073]The flow of culture gas and medium is shown in the figure. This model can simulate the influence of the same culture medium and culture gas on different types of human cells at the same time, such as studying the influence of PM2.5 pollutant exposure on various human tissues and organs. The specific implementation scheme is as follows: parallel connection from left to right The first single cell culture device, the second single...

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Abstract

The invention belongs to the fields of animal cell culture methods and manufacturing of culture equipment and particularly relates to a dynamic cell culture device and a single-species cell culture device. The dynamic cell culture device comprises a cell culture module, a culture medium supply module, a culture gas supply module and a circulating pump. According to the dynamic cell culture device, approximate-5D culture of cells can be realized aiming at a single-species cell culture situation; and aiming at a multi-species cell culture condition, the independent culture advantage of single-species cells is considered, and the independent culture and environment sharing of various species of cells are realized.

Description

technical field [0001] The invention belongs to the field of animal cell culture method and culture equipment manufacturing, and in particular relates to a cell dynamic culture device and a single cell culture device. Background technique [0002] Cell culture refers to a method of simulating the internal environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to enable it to survive, grow, reproduce and maintain its main structure and function. Cell culture technology can transform a single cell into simple single cell or rarely differentiated multi-cell through mass culture, which is an essential part of cloning technology, and cell culture itself is cell cloning. Cell culture technology is an important and commonly used technology in cell biology research methods. Through cell culture, a large number of cells can be obtained, and it can also be used to study cell signal transduction, cell anabolism, and cell growth and prolifera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
CPCC12M29/00C12M25/02C12M23/58C12M23/34C12M29/18C12M37/04
Inventor 胡伊乐杨为民卫志强李伟李三强杜珂珂杨晖
Owner HENAN UNIV OF SCI & TECH
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