High-purity stem cell culture device

A stem cell culture, high-purity technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve the problem that the number of cells cannot meet the clinical needs, the clinical application of stem cell therapy is difficult, and the influence of cell growth Larger and other problems, to achieve the effect of shortening the growth configuration cycle, improving the convenience of operation, and optimizing the cultivation parameters

Inactive Publication Date: 2018-12-28
曾小敏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both cell therapy and tissue engineering require a considerable number of stem cells. However, the source of stem cells is limited. The current planar culture operations such as ordinary multi-well plates and square flasks are complicated. The intermittent changes in the growth microenvironment have a great impact on cell growth. The number of harvested cells Far from meeting the clinical needs
At the same time, directly adding cytokine combinations to the culture system will greatly increase the cost of culture, making it difficult to promote stem cell therapy to clinical application

Method used

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  • High-purity stem cell culture device
  • High-purity stem cell culture device
  • High-purity stem cell culture device

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Such as Figure 1-8 As shown, the high-purity stem cell culture equipment includes a perfusion bottle 1, the perfusion bottle 1 is connected to the reactor 12 through the input pipe 6, the reactor 12 is connected to the peristaltic pump 8 through the first connecting pipe 7, and the peristaltic pump 8 is connected through the second connecting pipe 9 is connected with the perfusion tank 14, the perfusion tank 14 is connected with the waste liquid bottle 11 through the output pipe 10, the reactor 12 and the perfusion tank 14 are all provided with a filter membrane 13, the input pipe 6, the first connecting pipe 7, the second connecting pipe 9. Shock absorbers 5 are provided on the output pipes 10. The waste liquid bottle 11 includes a bottle body 11c with a multi-layer filter screen 11b inside the bottle body 11c, and a sterilizing lamp 11a on the outside of the bottle body 11c. A filter screen 11b is provided in the waste liquid bottle 11 to separate the solid matter in...

Embodiment 2

[0036] When the high-purity stem cell culture equipment of the present invention is actually used, the culture medium for stem cell culture is packed in the perfusion bottle 1, and the culture medium in the perfusion bottle 1 flows into the reactor 12 through the peristaltic pump 10 to serve as the filter membrane in the reactor 12. The feeder cells below 13 provide nutrition, and the embryonic stem cells are inoculated in the perfusion tank 14. The feeder cells in the reactor 12 secrete cytokines and culture fluid to form a conditioned medium, which is input into the perfusion tank 14 for embryonic stem cell culture and embryonic stem cell growth. Afterwards, the leftover culture medium is input into the waste liquid bottle 11 for collection.

[0037] In the initial stage of cell culture, the magnetic field intensity of the magnetic field generator 16 is controlled to be relatively small, so that the magnetic spheres 15 are suspended in the lower part of the reactor 12 or the ...

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Abstract

The invention discloses a high-purity stem cell culture device. The culture device comprises a perfusion bottle. The perfusion bottle is connected to a reactor through an inlet pipe, the reactor is connected to a peristaltic pump through a first communication pipe, the peristaltic pump is connected to a perfusion tank through a second communication pipe, and the perfusion tank is connected to a waste liquid bottle through an outlet pipe. A filter membrane is arranged in the reactor and the perfusion tank respectively, and a shock absorber is arranged on the inlet pipe, the first communicationpipe, the second communication pipe and the outlet pipe respectively. The waste liquid bottle includes a bottle body with a multilayer filter screen, and a sterilization lamp is arranged outside the bottle body. By means of the device, independent culture of stem cells can be achieved, the stem cell culture purity is high, the cell characteristics are reserved, and the culture process can be monitored.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a high-purity stem cell culture equipment. Background technique [0002] Stem cells are considered ideal seed cells for cell therapy and tissue engineering because of their self-renewal and multi-directional differentiation potential. Both cell therapy and tissue engineering require a considerable number of stem cells. However, the source of stem cells is limited. The current planar culture operations such as ordinary multi-well plates and square flasks are complicated. The intermittent changes in the growth microenvironment have a great impact on cell growth. The number of harvested cells It is far from reaching the clinical needs. At the same time, directly adding a combination of cytokines to the culture system will greatly increase the cost of culture, making it difficult to promote stem cell therapy to clinical application. Therefore, there is an urgent ne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/42C12M1/36C12M1/38
CPCC12M35/02C12M41/12C12M41/26
Inventor 曾小敏
Owner 曾小敏
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