Hydrophobic needle essence containing stem cell repair factors and preparation method of hydrophobic needle essence
A technology of stem cell factor and repair factor, applied in the field of smear-type water light needle essence containing stem cell repair factor and its preparation, which can solve the problems of limited skin effect and the like
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specific Embodiment example 2
[0031] Six Kunming mice were purchased from the Experimental Animal Center of China Medical University, 3 months old, weighing (30±4) g. Under sterile conditions, after anesthetized with 10% chloral hydrate (3.0 ml / kg), the back hair was depilated. A constant temperature and constant pressure scald apparatus was used to injure for 8 seconds. During the injury process, an 80°C cylindrical iron head with a bottom area of 1.5×1 cm and a mass of 0.5 kg was vertically contacted with the skin of the mouse, resulting in a deep II° wound. A total of six wounds were divided into two groups. The water light needles containing stem cell repair factors were applied externally [group A (n=3)], and the common water light needles on the market (without stem cell factors) [group B (n=3)] 3)].
[0032] Wound photography was performed on the first day (d1), seventh day (d7), fourteenth day (d14) and twenty-first day (d21) after the mice were burned, and according to the unhealed scale of the...
specific Embodiment example 3
[0037] skin moisture test
[0038] 1. Test material: the smear-type moisturizing needle produced in Example 1.
[0039] 2. Test subjects: healthy skin test subjects with no history of cosmetic allergies were selected, aged 18-45, including 30 males and 30 females, divided into 6 groups on average.
[0040] 3. Test method: The test is carried out under the conditions of room temperature 26°C±1°C and humidity 45%±5%. The test subjects first cleaned their faces for the same length of time (cleaning with clean water first, and then thoroughly cleaning with the same brand of facial cleanser). After the skin dried naturally, they selected the skin of 5cm×5cm on the cheeks as the test area, and took 1mL of the product prepared in Example 1. The water light needle essence is evenly applied to the test area. After using the product for 15 minutes, use the skin moisture tester of German CK Company to test, and measure the skin moisture content after 10 groups, 50 minutes, 100 minutes,...
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