Micro-nano biological particle enrichment and separation device based on acoustophoresis and oscillatory flow effects

Abstract

The invention relates to a micro-nano biological particle enrichment and separation device based on the acoustophoresis and oscillatory flow effects is ingenious in design, easy to operate, efficient and biologically friendly. A valveless micropump is used for generating oscillatory flow, the design of the oscillatory flow realizes infinite length of a short channel, and acoustic radiation force and inertial lift force are used for driving particles to a narrow enough micro-strip so as to realize enrichment and separation. Compared with single acoustophoresis control, the design has the advantages that the flux is higher, only a shorter straight channel is needed, the sound field intensity is low, and the operation is mild; compared with single inertial control, the design can maintain a lower level of shear stress, can reduce the risk of state change and activity loss of biological particles to a greater extent, and avoids the high-strength manufacturing requirement of a micro-fluidic chip at the same time. The device is simple in structure and high in integration level, can be used for efficient enrichment and separation of rare micro-nano biological particles such as circulating tumor cells and exosomes, and provides a platform for basic medical research and clinical rapid detection application.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, and in particular relates to a microfluidic device for enriching and separating micro-nano biological particles by utilizing sound field and oscillating flow effects. Background technique [0002] The control and treatment of micro-nano biological particles are commonly used in medical testing, biochemical reactions, environmental purification and other applications. The enrichment and separation of micro-nano biological particles is a key step, and has important applications in the fields of biology, medicine, chemistry and the environment significance. In recent years, since the control scale of microfluidic technology can match that of micro-nano particles, the development of many particle enrichment and separation technologies in microfluidics has been promoted. However, the existing methods are still difficult to achieve precise and efficient manipulation of micro-nano biological...

AI Technical Summary

Problems solved by technology

However, like other active methods, acoustophoretic manipulation also often requires a longer sound field action time, which means that a longer flow channel is required, resulting in increased costs; of course, the sound field intensity is usually increased to shorten the action time, but Under the action of high-intensity sound field, cell viability is impaired and mechanobiological effects are unavoidable, which limits the practical application of acoustophoretic manipulation to a certain extent.

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