Optical chromatography microscopic imaging system and method for rapidly and continuously rotating sample

Abstract

The invention discloses an optical chromatography microscopic imaging system and an optical chromatography microscopic imaging method for rapidly and continuously rotating a sample. Optical tweezers manufactured by linear polarization are used for capturing and rotating a sample, a single-beam gradient force trap is generated, microparticles or cells can be restrained near a focus in a three-dimensional space, anisotropic polarization in the sample is caused at the same time, and therefore a moment depending on an included angle between a birefringence polarization direction and an electric field direction is generated, the captured object can be controlled to synchronously rotate with the polarization direction of the light field. An optical heterodyne interference method is adopted to synthesize a polarized high-speed rotating linear polarized light field for generating optical tweezers, a target sample can be rapidly and continuously rotated in a non-contact mode, mechanical disturbance introduced by a scanning galvanometer is avoided, and especially for optical diffraction tomography, the axial resolution can be improved, and isotropy of the resolution can be achieved. The optical path is compact, and the miniature optical path can be realized.

Description

Technical field [0001] The present invention relates to optical imaging microscopy, particularly relates to optical tomographic imaging microscopic imaging system and a method of rotating the sample in rapid succession. Background technique [0002] Using an optical microscope interaction of light and matter to achieve fine objects to the human eye can not distinguish, for example, cells, and amplifying a technical means for imaging. Compared to other imaging modalities such as electron microscopy microscope, an optical microscope can be achieved noninvasive living cells in real time imaging and observation, it has a wide range for biomedical application scenarios. [0003] The optical tomographic imaging microscopy using light scattered by the sample, or the excitation of the fluorescence signal transmission to perform tomographic imaging of the sample, thereby reconstructing a three-dimensional digital model of imaging on the sample. Wherein the optical projection tomography (O...

AI Technical Summary

Problems solved by technology

However, the existing rotating sample technology is usually realized by mechanical means, and it also has some inherent defects: (1) the speed of mechanical rotation limits the time resolution of imaging; (2) the capillary microtube as the mechanical rotating arm The reflection needs additional correction of numerical aperture; (3) The means on the mechanical rotation sample will cause deformation on the biological sample due to the contact with the sample

While this rotation on the sample is usually mechanical contact, there are the same disadvantages and effects in optical diffraction tomography

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