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Biomarker method for identifying existence form of human pituitary tissue lactation hormone protein

A biomarker, protein technology, applied in the field of molecular biology

Pending Publication Date: 2021-12-03
SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Despite the availability of multiple treatments including drug therapy and surgical resection, a series of PRL-associated diseases caused by pituitary tumors remain an important clinical problem

Method used

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  • Biomarker method for identifying existence form of human pituitary tissue lactation hormone protein
  • Biomarker method for identifying existence form of human pituitary tissue lactation hormone protein
  • Biomarker method for identifying existence form of human pituitary tissue lactation hormone protein

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Embodiment 1

[0025] The biomarker method for identifying the form of prolactin protein in human pituitary tissue in this embodiment is as follows:

[0026] S1. Collect pituitary adenoma and normal pituitary tissue samples, lyse and extract the tissues respectively, and obtain pituitary adenoma tissue protein and normal pituitary tissue protein;

[0027] Human control normal pituitary tissue samples were postmortem tissue obtained from the National Center for Disease Communication and Memphis Regional Medical Center under the approval of the University of Tennessee Health. Human pituitary adenoma tissue was obtained from Emory University Hospital, and with the approval of the university, all tissues were removed and stored in liquid nitrogen until processing;

[0028] The specific protein extraction methods are:

[0029] Each tissue sample (about 600 mg) was washed with 0.9% sodium chloride solution (3 ml, 5 times) with a mass fraction to completely remove blood, and finely grind the tissu...

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Abstract

The invention provides a biomarker method for identifying the existence form of human pituitary tissue lactation hormone protein. The method comprises the following steps of collecting pituitary adenoma and normal pituitary tissue samples, respectively extracting the tissue proteins, scanning a visual PVDF membrane and bidirectional gel into a digital image through bidirectional gel electrophoresis and western blot silver staining, digesting and purifying the corresponding two-way gel protein spots, and identifying the difference of the existing form proportions of different pituitary tissue lactation hormone proteins of different pituitary tumor sub-types through mass spectrum identification and bioinformatics analysis. According to the present invention, the existence forms of the human pituitary tissue lactation hormone protein and the differential expression profiles of the prolactin proteins in different pituitary tumors are identified, so that the development of drugs for blocking prolactin signal channels for clinical treatment is facilitated.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a biomarker method for identifying the existence form of prolactin protein in human pituitary tissue. Background technique [0002] Pituitary tumors are one of the most common primary intracranial tumors, accounting for 17% of intracranial tumors. Abnormal secretion of prolactin (PRL) by pituitary tumors can lead to clinical diseases, such as hyperprolactinemia. Despite the availability of various treatments such as drug therapy and surgical resection, a series of PRL-related diseases caused by pituitary tumors are still important clinical problems. Therefore, searching for biomarkers of prolactin proteoform (PRLP) in pituitary tumors and PRLP biomarkers in related prolactin diseases is one of the key means to deal with pituitary tumors and prolactin-related diseases. Contents of the invention [0003] The technical problem to be solved by the present in...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/574G01N27/62
CPCG01N33/74G01N33/6848G01N33/57484G01N33/57407
Inventor 詹显全钱社华王小伟
Owner SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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