Antibacterial peptide and application thereof
A technology of antimicrobial peptides and compositions, applied in the fields of antimicrobial peptides and their applications, which can solve the problems of unknown effects of antimicrobial peptides, damage to the ecology of oral flora, lack of evaluation of antimicrobial peptide strain regulation, etc., to achieve drug resistance, good Biosecurity Effects
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[0041] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific examples.
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[0043]
[0044]
[0045] 1. Solid-phase synthesis of antimicrobial peptides
[0046] The preparation of antimicrobial peptide of the present invention adopts chemical solid-phase synthesis technology, and concrete preparation method is as follows:
[0047](1) Use Fmoc-His(Trt)-Wang Resin as the starting material, add dichloromethane to fully swell the resin, and then use hexahydropyridine / N,N-dimethylformamide (DMF) solution (DBLK solution) Remove the 9-fluorenylmethoxycarbonyl (Fmoc) protecting group, and wash several times with DMF, and clean up the remaining DBLK solution;
[0048] (2) Then, the remaining 11 amino acids constituting the antimicrobial peptide were sequentially synthesized in solid phase using the Fmoc strategy supplemented by ...
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