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Micro-fluidic chip for tumor cell cluster sorting

A microfluidic chip and tumor cell technology, applied in the field of microfluidic chips, can solve the problems of sensitivity to cell shape, structure and size, fluid disturbance deviating from a preset focus position, low separation accuracy, etc. The effect of high distance and flux

Pending Publication Date: 2021-11-30
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, inertial microfluidic technology is sensitive to the shape, structure and size of cells, and it is easy to be disturbed by the fluid and deviate from the preset focus position when sorting cluster cells and abnormal cells.
Although the common helical inertial microfluidic chip used for cell separation can achieve certain separation purposes, its separation accuracy is lower than that of the active type.

Method used

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  • Micro-fluidic chip for tumor cell cluster sorting
  • Micro-fluidic chip for tumor cell cluster sorting
  • Micro-fluidic chip for tumor cell cluster sorting

Examples

Experimental program
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Embodiment Construction

[0026] The technical solution of the present disclosure will be described in detail below in conjunction with the accompanying drawings.

[0027] figure 1 It is a schematic structural diagram of the microfluidic chip described in this application, such as figure 1 As shown, the microfluidic chip includes an upper cover, a channel layer and a lower cover. As a specific embodiment, the upper cover is a PET film, the channel layer is made of silica gel, and the lower cover is a PVC cover.

[0028] The structure of the channel layer is as figure 2 As shown, the flow channel layer includes a sample inlet 1, a spiral and sudden expansion coupling flow channel 2, a collection port 3 and a waste liquid port 4, and the spiral and sudden expansion coupling flow channel 2 is in a spiral shape.

[0029] The injection port 1 is located at the beginning of the helical and burst coupling flow channel 2, and communicates with the outside world through a conduit.

[0030] The spiral and su...

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Abstract

The invention discloses a micro-fluidic chip for tumor cell cluster sorting, relates to a cell cluster sorting device, and solves the technical problems of low control precision and low flux of the existing micro-fluidic chip. According to the technical scheme, a spiral main flow channel of the micro-fluidic chip is designed into a trapezoidal cross section, and compared with a rectangular flow channel structure, asymmetric secondary flow vortexes perpendicular to the main flow direction of the cross section are generated. Cells gradually generate an inertial focusing effect under the influence of inertial lift force and secondary flow Dean drag force and transversely migrate to different balance positions. Specifically, small-size monomer cells are trapped to a strong vortex close to the outer wall surface of the flow channel under the dominant action of strong secondary flow, and large-size cluster cells are focused on the inner wall surface of the flow channel under the dominant action of strong inertial lift force, so that the separation of monomer cells and cluster cells with different sizes can be realized.

Description

technical field [0001] The present application relates to a sorting device for cell clusters, in particular to a microfluidic chip for sorting tumor cell clusters. Background technique [0002] Tumor metastasis is the main cause of death of cancer patients, and early diagnosis and treatment are of vital importance to improve the survival rate of cancer patients. The steps of tumor metastasis include: tumor cells detach from the tumor lesion, enter the blood circulation, invade distant organs, and form metastases. Circulating tumor cells (CTCs) refer to tumor cells released from the primary or metastatic sites of solid malignant tumors into the peripheral blood circulation, which play an important role in the process of tumor metastasis. There are also clusters of circulating tumor cells (CTC clusters) in the peripheral blood of some tumor patients, which are formed by the aggregation of two or more circulating tumor cells. Compared with single tumor cells, tumor cell cluste...

Claims

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Application Information

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IPC IPC(8): B01L3/00C12M1/00
CPCB01L3/502761C12M23/16C12M23/38
Inventor 项楠任慧倪中华
Owner SOUTHEAST UNIV
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