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DNA breaking instrument

A water tank and electrode array technology, which is applied in the field of sample pretreatment instruments in the field of molecular biology, can solve the problems that the uniformity of the interrupted DNA reaction and low temperature requirements cannot be well met, and achieves simple structure, reasonable cost, and uniformity of reaction. Good results

Pending Publication Date: 2021-11-23
杭州奥明医学检验实验室有限公司
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  • Description
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Problems solved by technology

[0004] The liquid-phase plasma method must be inseparable from the DNA-interrupting device of the liquid-phase plasma method, but there is almost no research on the plasma processing device for DNA-interrupting at this stage, and the existing experimental devices are not very good. Meet the reaction uniformity and low temperature requirements required for DNA fragmentation

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Embodiment 1

[0017] The DNA breaking instrument of embodiment 1, such as figure 1 As shown, it includes a case 1, a water tank 3 with a shell 2 is installed on the case 1, a sample tank 5 for placing a centrifuge tube 4 and an inverted L-shaped connecting rod 7 and 8 connecting the sample tank 6 are installed at the front and rear ends of the water tank 3 , the top of the shell 2 of the water tank is the coolant inlet 9, the bottom of the shell 2 of the water tank is the coolant outlet 10, the coolant inlet 9 is connected to the electronic peristaltic pump 11, and the electronic peristaltic pump 11 is connected to a cylindrical temperature control system 12 Connected, the temperature control system 12 includes a computer temperature controller 13, a shell temperature sensor 14 and a water tank temperature sensor 15 of the water tank.

[0018] In embodiment 1, the liquid phase plasma assembly of DNA fragmentation instrument, such as figure 1 As shown, it includes a power supply 16 , a cont...

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Abstract

The invention discloses a DNA breaking instrument. According to the DNA breaking instrument, a water tank with a shell layer is mounted on a machine box of the DNA breaking instrument, a temperature control system capable of accurately controlling the temperature is connected to the shell layer of the water tank, L-shaped sample seat assemblies for placing sample tanks are mounted on the front side and the rear side of the water tank, the sample seat assemblies are foldable, when the sample seat assemblies are unfolded, sample tubes in the mounted sample tanks can be suspended in the water tank, microelectrode arrays are installed on the left side and the right side of the machine box through assemblies, and the two microelectrode arrays are connected with a power source and a controller of a liquid phase plasma device; and when the breaking instrument is used, the sample seat assemblies are unfolded, the sample tubes are placed on the sample tanks, water is filled in the water tank, and a centrifugal tube is in contact with the water, so that non-contact liquid-phase plasma of a sample in the sample tube can be broken. The breaking instrument is simple in structure and wide in application, is mainly used for breaking DNA required by sequencing, and can also be used for preparing DNA and DNA terminal protein compounds and the like.

Description

technical field [0001] The invention relates to a sample pretreatment instrument in the field of molecular biology, in particular to a liquid-phase plasma-based DNA fragmentation instrument. Background technique [0002] Plasma, also called plasma, is the fourth state of matter except solid, liquid, and gas. Through cleverly designed electromagnetic fields, people can make full use of the energy generated by the plasma. General plasma refers to gas phase plasma. In order to distinguish gas-phase plasma, the plasma generated by electrode discharge in solution is called liquid-phase plasma. [0003] Currently, there are mainly two methods of DNA fragmentation: contact and non-contact. The contact method mainly includes crushing method and enzymatic cutting method; the non-contact method mainly refers to the ultrasonic breaking method using ultrasonic pulverization technology. These methods all have certain limitations. For example, the contact method has certain deficienci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/34C12M1/24C12M1/02C12M1/00
CPCC12Q1/6806B01J19/08C12Q2523/30C12Q2535/122
Inventor 唐正清王华印赵楠
Owner 杭州奥明医学检验实验室有限公司
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