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Preparation method of floatable microalgae particles

A particle and microalgae technology, applied in the field of preparation of microalgae immobilized particles, can solve problems such as particle modification not mentioned, and achieve the effects of enhancing the utilization efficiency of light energy, increasing the contact area, and reducing the energy consumption of mixed aeration

Pending Publication Date: 2021-11-12
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Invention patent application CN201810301952.5 discloses a method for treating aquaculture wastewater with immobilized chlorella. The immobilized chlorella is used to treat aquaculture wastewater, achieving a good removal effect on nitrogen and phosphorus; patent application CN201810058472.0 discloses A microalgae algae ball and its immobilization method, only related to the immobilization method and the method of dissolving and harvesting; patent application CN201711487241.3 discloses a method for microalgae semi-solidified polyculture to treat fracturing flowback fluid, and activated carbon powder and Mixing microalgae for immobilization treatment of fracturing backflow fluid; the above patent documents all mention that immobilized microalgae particles are used to treat sewage and are applied in different scenarios, but the transformation of particles is not mentioned

Method used

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  • Preparation method of floatable microalgae particles

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preparation example Construction

[0031] Embodiments of the present invention provide a method for preparing floating microalgae particles, comprising the following steps:

[0032] S1. Mixing the microalgae cell suspension with the sodium alginate solution to obtain an initial mixed solution;

[0033] S2. Drop the initial mixed solution into the calcium chloride solution to form hydrogel particles, and keep stirring to obtain floatable microalgae particles through cross-linking and solidification reaction;

[0034] Wherein, the density of the microalgae particles is regulated to be smaller than the density of the culture water body (or culture substrate), so that the microalgae particles can float on the water surface. The culture water body is mainly the water body containing various nutrients such as nitrogen and phosphorus, such as domestic sewage, microalgae nutrient solution, etc.

[0035] In the present invention, the preparation of the floating microalgae particles is realized by adjusting the density ...

example 1

[0068] EXAMPLE 1: MICROALGAL PELLETS CONDITIONING AIR AIR BUBBLES IN THE PELLETS

[0069] Continuously blow air into the initial mixed solution of microalgae sodium alginate and make it evenly distributed to ensure that the volume of gas in the initial mixed solution during the dropping process is about one-fifth of the particle volume; drop the initial mixed solution into chlorine Calcium chloride solution forms hydrogel particles, which are constantly stirred, and cross-linked and solidified for more than half an hour. After the particles are solidified, they are washed with water for 2 to 3 times and then put into the culture medium for 3 days to harvest.

[0070] Experimental tests show that the prepared microalgae particles can float on the water surface, such as figure 1 shown.

example 2

[0071] Example 2: Added Microalgae Particles to Adjust Foaming Agents

[0072] Fully mix the foaming agent polyvinyl alcohol and the initial mixed solution by stirring to form a foaming agent mixed solution; drop the foaming agent mixed solution into the cross-linking solution with boric acid as an auxiliary cross-linking agent, and keep stirring to cross-link The solidification reaction lasts for more than half an hour, and then the particles are solidified and washed with water for 2 to 3 times, then put into the culture medium for 3 days and harvested. According to experimental tests, the foaming agent helps the air to stay and evenly distribute in the particles, so that the prepared microalgae particles can float on the water surface better.

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Abstract

The invention relates to a preparation method of floatable microalgae particles. The preparation method comprises the following steps: S1, mixing a microalgae cell suspension with a sodium alginate solution to prepare an initial mixed solution; and S2, dropwise adding the initial mixed solution into a calcium chloride solution to form hydrogel particles, continuously stirring, and carrying out a cross-linking curing reaction to obtain the floatable microalgae particles, wherein the density of the microalgae particles is regulated and controlled to be smaller than the density of a culture medium, so that the microalgae particles can float on the water surface. The microalgae particles can float on the water surface by adjusting the density of the microalgae particles, the prepared microalgae can grow and breed by fully utilizing illumination, carbon dioxide and nutrient substances such as carbon, nitrogen and phosphorus in water, and further biomass extraction is facilitated. Therefore, the energy consumption requirement of stirring or aeration can be reduced, full utilization of light energy is promoted, the defects caused by microalgae sedimentation are reduced, and meanwhile, a large amount of microalgae can be harvested conveniently.

Description

technical field [0001] The invention relates to the field of microalgae cultivation, in particular to a preparation method of microalgae immobilized particles. Background technique [0002] Microalgae can efficiently absorb nutrients such as carbon, nitrogen, and phosphorus in water, providing a solution for improving water quality. The immobilized culture of microalgae has the advantages of reducing the risk of bacterial contamination, improving the efficiency of cultivation and harvesting, and reducing energy consumption. Using immobilized microalgae particles to treat sewage while cultivating microalgae has become a promising culture method. By adjusting the floating ability of the microalgae particles, the microalgae particles are allowed to float on the water surface, which is beneficial to the efficient use of light energy by the microalgae, and at the same time makes it easier to absorb carbon dioxide in the air, which is beneficial to the cultivation and harvest of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/04C12R1/89
CPCC12N11/10C12N11/04
Inventor 陶益曹松滕飞
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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