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Fermented traditional Chinese medicine oral liquid for treating piglet diarrhea and improving its intestinal flora

A technology of traditional Chinese medicine oral liquid and intestinal flora, applied in antibacterial drugs, drug combination, drug delivery, etc., can solve problems such as endogenous infection of animals, destruction of micro-ecological balance, mutation of drug-resistant bacteria, etc., to achieve therapeutic Effects of diarrhea in piglets, improvement of growth performance, stimulation of immune response

Active Publication Date: 2022-05-06
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term and large-scale use of antibiotics has destroyed the micro-ecological balance of the body, leading to the emergence of drug-resistant bacteria and bacterial mutations
Seriously affect the survival rate of piglets, and easily cause animal endogenous infection or secondary infection

Method used

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  • Fermented traditional Chinese medicine oral liquid for treating piglet diarrhea and improving its intestinal flora
  • Fermented traditional Chinese medicine oral liquid for treating piglet diarrhea and improving its intestinal flora
  • Fermented traditional Chinese medicine oral liquid for treating piglet diarrhea and improving its intestinal flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: The preparation of this treatment of piglet diarrhea and improvement of its intestinal flora fermented traditional Chinese medicine oral liquid

[0020] (1) Resuscitate the strains preserved in a volume ratio of 50% glycerol:bacteria solution=7:3 in a water bath at 37°C for 1 min, and culture the revived Lactobacillus salivarius, Lactococcus lactis, and Lactobacillus plantarum in the MRS with an inoculation loop Streak on the base, streak the Bacillus licheniformis species on the LB medium with an inoculation loop, and then place it in a 37°C constant temperature incubator for 12 hours; pick a single colony and inoculate it into a sterile liquid medium, and Cultured on a shaker to OD 600 The value is 0.6~0.8, subculture the bacterial solution with 1% inoculum, and then inoculate the subcultured bacterial solution into the required amount of sterile liquid medium for cultivation at 37°C to obtain an effective viable count of 10 8 ~10 9 CFU / mL Lactobacillus s...

Embodiment 2

[0024] Example 2: The preparation of this treatment of piglet diarrhea and improvement of its intestinal flora fermented traditional Chinese medicine oral liquid

[0025](1) Resuscitate the strains preserved in a volume ratio of 50% glycerol:bacteria solution=7:3 in a 37°C water bath for 1 min, and use an inoculation loop to inoculate the strains of Lactobacillus salivarius, Lactococcus lactis, and Lactobacillus plantarum Streak on the MRS medium, use an inoculation loop to streak the Bacillus licheniformis species on the LB medium, and then place it in a constant temperature incubator at 37 ° C for 12 hours; pick a single colony and shake it in a sterile liquid medium bed culture to OD 600 value of 0.6~0.8, subculture the bacterial solution with 1% inoculum, and then inoculate the subcultured bacterial solution into a sterile liquid medium for cultivation at 37°C to obtain an effective viable count of 10 8 ~10 9 CFU / mL Lactobacillus salivarius liquid, effective number of vi...

Embodiment 3

[0029] Example 3: Tolerance of 4 strains in the compound probiotic bacterial liquid to simulated gastrointestinal fluid

[0030] Preparation of artificial gastric juice: Take 1.64mL of 1mol / L dilute hydrochloric acid in a container, add 80mL of water, add 1g of pepsin, adjust the pH to 3.0, mix well and set the volume to 100mL, sterilize with a 0.22μm filter membrane; prepare pH4.0 in the same way Artificial gastric juice, placed in a 4°C refrigerator for later use;

[0031] Preparation of artificial intestinal juice: Take 0.68g of potassium dihydrogen phosphate, add 80mL of water to dissolve, add 1g of trypsin, adjust the pH to 6.8 with 0.1mol / L sodium hydroxide solution; Make simulated intestinal juice and store it in a 4°C refrigerator for later use;

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Abstract

The invention discloses a fermented traditional Chinese medicine oral liquid for treating piglet diarrhea and improving its intestinal flora. Mix 0.6kg of poplar flowers evenly, adjust the water content to 55-65%, and ferment the traditional Chinese medicine at 37°C for 6-8 days to complete the fermentation of traditional Chinese medicine. 0.5-0.7L of deionized water, after mixing, stand at 23-26°C for 24-30 hours of static soaking and extraction, stir irregularly during the standing soaking, and then filter through a filter to remove the filter residue to obtain the fermented Chinese medicine oral liquid; the fermented Chinese medicine of the present invention The oral solution fully exerts the effects of probiotics, traditional Chinese medicine and its fermentation products, can significantly treat piglet diarrhea, improve the intestinal flora of piglets, reduce bacterial resistance and antibiotic drug residues caused by the abuse of antibiotics in feed, and ensure food production safety; The preparation method of the fermented traditional Chinese medicine oral liquid of the invention is simple, and is suitable for industrial production and market promotion and application.

Description

technical field [0001] The invention belongs to the technical field of compound probiotic preparations, and in particular relates to a fermented traditional Chinese medicine oral liquid for treating piglet diarrhea and improving its intestinal flora. Background technique [0002] Piglets are the core of modern pig production, and their health status has an extremely important impact on later growth and development. Since the piglet's own immune system is not fully developed, the invasion of harmful microorganisms can easily cause diarrhea in piglets. Piglets within one month of birth, due to incomplete development of digestive glands and thermoregulatory centers, such as temperature changes and excessive milk fat, can easily cause indigestion and diarrhea. Diarrhea is a common disease in pig farms, which can lead to a decrease in the survival rate of piglets and affect the composition of intestinal flora. Abnormal intestinal flora can also easily cause diarrhea and bring hu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/08A61K36/884A61P1/12A61P1/00A61P31/04A61K35/747A61K35/744A61K35/742
CPCA61K36/484A61K36/718A61K36/282A61K36/752A61K36/734A61K36/346A61K36/355A61K36/233A61K36/288A61K36/855A61K36/704A61K36/315A61K36/195A61K36/78A61K36/539A61K36/744A61K36/536A61K36/284A61K36/756A61K36/884A61K36/076A61K36/481A61K35/747A61K35/744A61K35/742A61K9/0095A61P1/12A61P1/00A61P31/04A61K2236/19A61K2300/00
Inventor 林连兵徐美余朱付千何秀邓先余张棋麟王峰郭军
Owner KUNMING UNIV OF SCI & TECH
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