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Application of amitriptyline to preparation of medicine for improving endothelial cell functions

A technology of endothelial cells and amitriptyline, which is applied in the field of medicine, can solve the problems of amitriptyline that have not been reported, and achieve the effect of alleviating the dysfunction of human umbilical vein endothelial cells, good application prospects, and restoring the ability of tube formation

Pending Publication Date: 2021-11-09
THE SECOND AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although existing studies have reported that amitriptyline plays a protective role in various diseases, it has not been reported that amitriptyline can reduce endothelial cell inflammation or improve endothelial function and its mechanism of action

Method used

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  • Application of amitriptyline to preparation of medicine for improving endothelial cell functions
  • Application of amitriptyline to preparation of medicine for improving endothelial cell functions
  • Application of amitriptyline to preparation of medicine for improving endothelial cell functions

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Experimental program
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Embodiment 1

[0032] 1. Experimental materials

[0033] Amitriptyline hydrochloride was purchased from Sigma Company of the United States; human recombinant TNF-α was purchased from Beijing Sino Biological Co., Ltd.; EBM-2 medium was purchased from Lonza Company, 1640 medium was purchased from Gibco Company of the United States; EdU kit was purchased from Guangzhou Rui Bo company; Matrigel was purchased from Corning Corporation of the United States; ASMase antibody was purchased from China Aibotec; ICAM-1, VCAM-1, MCP-1, MAPK, p-MAPK, p-NF-κB and other antibodies were purchased from CST Corporation of the United States ; ICAM-1 and VCAM-1 Elisa kits were purchased from China Dakowei Company.

[0034] 2. Experimental cell culture and model establishment

[0035] Human umbilical vein endothelial cells (Human Umbilical Vein Endothelial Cell, HUVEC) were purchased from Cell Applications, USA.

[0036] Cells were cultured in 95% air and 5% CO 2 in a 37°C sterile incubator. When the cells wer...

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Abstract

The invention discloses an application of amitriptyline to preparation of a medicine for improving endothelial cell functions, and belongs to the field of medicines. The medicine for improving the endothelial cell functions comprises any one of the following medicines: a medicine for promoting eNOS phosphorylation and NO release in endothelial cells, a medicine for inhibiting an endothelial cell inflammatory response induced by TNF-alpha, a medicine for inhibiting generation of endothelial cell active oxygen induced by TNF-alpha, and a medicine for relieving endothelial cell inflammation by inhibiting an MAPK signal channel and activation of NF-kappa B. The invention discloses and points out an action mechanism of the amitriptyline in vascular endothelial injury and inflammatory response for the first time, and tests verify that the amitriptyline can inhibit the vascular endothelial injury and the inflammatory response so as to improve endothelial cell functions. The amitriptyline has a good application prospect for preventing cardiovascular diseases caused by endothelial cell inflammation and endothelial cell dysfunction, and opens up a new medical approach for preventing and treating cardiovascular diseases.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of amitriptyline in the preparation of drugs for improving the function of endothelial cells. Background technique [0002] Atherosclerosis (atherosclerosis, AS) is the main cause of cardiovascular disease (cardiovasculardisease, CVD). AS is a chronic inflammatory disease of arterial intima, and its pathological changes can be divided into three stages: fatty streak formation, fibrous plaque and atherosclerotic plaque. At various stages, blood components, blood vessel wall constituent cells and extracellular matrix interact, including endothelial insufficiency, lipoprotein infiltration and modification, leukocyte aggregation, proliferation and migration of vascular smooth muscle cells, foam cell formation and extracellular matrix deposition, etc., causing The thickening and hardening of the arterial wall, stenosis or occlusion of the lumen lead to the decline of the arter...

Claims

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Application Information

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IPC IPC(8): A61K31/135A61P9/10A61P9/00A61P29/00
CPCA61K31/135A61P9/10A61P9/00A61P29/00
Inventor 钟赟季杨陈静叶锦豪陈昌浓陈换珍
Owner THE SECOND AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV
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