Method for inducing hairy roots of stylosanthes guianensis

A technique for styli and hairy roots, which is applied in the field of hairy root induction of styli, and can solve the problems of low transgenic efficiency, complexity, and no reports of styli hairy roots.

Pending Publication Date: 2021-11-05
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the whole plant transformation method to obtain transgenic materials has disadvantages such as a relatively complicated transgenic process, low transgenic efficiency, and long transformation time, which is not conducive to the study of the gene function of Stylophyllum.
Therefore, the invention of the method for inducing the hairy root of Stylophyllum is of great significance for the study of the gene function of Stylophyllum, but there is no report about the induction of the hairy root of Stylophyllum

Method used

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  • Method for inducing hairy roots of stylosanthes guianensis
  • Method for inducing hairy roots of stylosanthes guianensis
  • Method for inducing hairy roots of stylosanthes guianensis

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Embodiment 1

[0031] 1. Method

[0032] 1. Culture medium configuration

[0033] (1) Germination medium (1×MS medium) consists of: macroelements: 1.65g L -1 NH 4 NO 3 , 1.9g·L - 1 KNO 3 , 0.44g·L -1 CaCl 2 2H 2 O, 0.37g L -1 MgSO 4 ·7H 2 O, 0.17g L -1 K H 2 PO 4 ;Trace element: 0.83mg·L -1 KI, 6.2 mg L -1 h 3 BO 3 , 22.3mg·L -1 MnSO 4 4H 2 O, 10.6mg·L -1 ZnSO 4 .7H 2 O, 0.25mg·L -1 Na 2 MoO 4 2H 2 O, 0.025mg·L -1 CuSO 4 ·5H 2 O, 0.025mg·L -1 CoCl 2 ·6H 2 O, 37.3mg·L -1 Fe-EDTA; 1% sucrose; 0.3% vegetable gum. The germination medium was sterilized at 121° C. for 20 minutes; after cooling to 60° C., the medium was poured into a square petri dish with a diameter of 13*13 cm.

[0034] (2) The configuration steps of Agrobacterium rhizogenes medium are as follows: YEP medium consists of: 10g L -1 Tryptone, 10g·L -1 Yeast extract, 5g·L -1 NaCl, 15g L -1 Agar (liquid medium does not need to be added). After preparation, sterilize in a sterilizing pot at 121°...

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Abstract

The invention discloses a method for inducing hairy roots of stylosanthes guianensis. The method comprises the steps of selection and culture of agrobacterium rhizogenes, acquisition of stylosanthes guianensis explants, infection of the stylosanthes guianensis explants, induction of the hairy roots of the stylosanthes guianensis and hairy root degerming culture and propagation, and the hairy root induction rate reaches 58.3%. The method for inducing the hairy roots of the stylosanthes guianensis provides important technical support for molecular biology research of the stylosanthes guianensis, and has a great application prospect.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, and more specifically, relates to a method for inducing hairy roots of Stylosmanthus. Background technique [0002] Stylosanthes guianensis is a perennial leguminous forage mainly distributed in tropical and subtropical regions. It has high yield and good quality, and is mainly used for animal feed and forest land greening. Stylo is an excellent pioneer crop for improving acidic soils. [0003] In nature, Agrobacterium rhizogenes (Agrobacterium rhizogenes) can stably integrate the hairy root-inducing rol gene into the host plant genome through the T-DNA of the Ri plasmid to produce hairy roots. The genes are co-integrated into the plant genome, thereby achieving co-transformation. The use of Agrobacterium rhizogenes to obtain transgenic plant hairy roots has been achieved in a variety of plants. For example, hairy roots obtained from plants such as Phaseolus vulgaris, Glycinemax, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/06A01H6/54
CPCC12N15/8205
Inventor 梁翠月林雁彭光粉郭雪琼陈倩倩
Owner SOUTH CHINA AGRI UNIV
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