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Method for extracting glycyrrhizic acid based on subcritical water extraction technology

An extraction technology, subcritical water technology, applied in the field of extracting glycyrrhizic acid based on subcritical water extraction technology, can solve the problems of low extraction rate of glycyrrhizic acid, large amount of use of organic solvents, etc., and achieve the effect of broad development and utilization prospects

Pending Publication Date: 2021-11-02
无锡市本草机械科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Purpose of the invention: In order to solve the above-mentioned technical problems, the present invention provides a method for extracting glycyrrhizic acid based on subcritical water extraction technology, which effectively solves the two problems of low extraction rate of glycyrrhizic acid during traditional extraction and the use of a large amount of organic solvents during extraction. big problem

Method used

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  • Method for extracting glycyrrhizic acid based on subcritical water extraction technology
  • Method for extracting glycyrrhizic acid based on subcritical water extraction technology
  • Method for extracting glycyrrhizic acid based on subcritical water extraction technology

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Effect test

Embodiment 1

[0038] Detection of the total content of glycyrrhizic acid: according to the method of the 2020 edition of "Chinese Pharmacopoeia", take 0.2 g of licorice powder (passed through a No. 3 sieve), put it in an Erlenmeyer flask, add 100 mL of 70% ethanol precisely, seal it, and sonicate for 30 minutes. The filtrate was passed through a 0.45 μm filter membrane for liquid phase testing, and the test result was the total content of glycyrrhizic acid in the purchased licorice. Comparing the liquid chromatogram of the standard product, the peak appears at the retention time of about 31min, and the total content of glycyrrhizic acid in licorice is calculated to be 17.85mg / g.

Embodiment 2

[0040] Orthogonal experiment was designed by factor rotation method to explore the influence of pressure on the extraction effect: take 30g of pretreated licorice, add 1600mL of pure water, the ratio of liquid to material is 53:1 (mL / g), heat, and stop heating after reaching 105°C , pressurize to 0.2, 0.5, 1.5, 2.5, 3Mpa respectively, heat preservation and pressure extraction for 30 minutes, turn on the cooling water after the extraction, condense the steam, then further cool down, and finally collect the extract. The extract was freeze-dried for 3 days, water was removed, dissolved in 70% ethanol, and passed through a 0.45 μm filter membrane for sampling and testing. Take the extraction effect as the evaluation index to measure the influence of pressure, such as image 3 shown.

Embodiment 3

[0042] Orthogonal experiment was designed by factor rotation method to explore the influence of liquid-material ratio on the extraction effect: add 1600mL of pure water, the extraction temperature is 105°C, the pressure is the optimal pressure in Example 1, that is, pressurize to 0.2Mpa, the amount of licorice 20, 26, 30, 35, 40g respectively, i.e. the ratio of liquid to material is 40:1~80:1 (mL / g), heat preservation and pressure extraction for 30 minutes, after the extraction is completed, turn on the cooling water to condense the steam, and then further cool down, Finally collect the extract. The extract was freeze-dried for 3 days, water was removed, dissolved in 70% ethanol, and passed through a 0.45 μm filter membrane for sampling and testing. Take the extraction effect as the evaluation index to measure the influence of the ratio of liquid to material, such as Figure 4 shown.

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Abstract

The invention discloses a method for extracting glycyrrhizic acid based on a subcritical water extraction technology. The method comprises the following steps: (1) pretreating liquorice; (2) taking the pretreated liquorice, and adding pure water or pure water containing a food-grade acid-base regulator; (3) heating and pressurizing to reach a water subcritical state; (4) extracting in a water subcritical state; and (5) after the extraction is finished, cooling, and collecting the extract liquor to obtain a product. The invention provides a green and efficient extraction process which takes water as a solvent, does not add any auxiliary material or any organic reagent and stabilizes the glycyrrhizic acid extraction rate at 90% or above, and effectively solves the two major problems that the glycyrrhizic acid extraction rate is low and a large amount of organic solvent is used during extraction in the traditional extraction process.

Description

technical field [0001] The invention belongs to the technical field of glycyrrhizic acid extraction, and more specifically relates to a method for extracting glycyrrhizic acid based on subcritical water extraction technology. Background technique [0002] Glycyrrhizic acid (Glycyrrhizic acid) is an effective active ingredient extracted from licorice roots and stems. It is a very precious natural saponin with antioxidant and adrenocorticotropic hormone-like activities. According to literature research, glycyrrhizic acid and its series of products can inhibit the growth of sarcoma and cancer cells, and the inhibition rate of AIDS is as high as 90%, which has a strong effect of increasing human immune function. And its sweetness is about 50 times that of sucrose, it is a low-calorie sweetener, and it is a good food additive and spice base. Therefore, glycyrrhizic acid has been widely used in medicine, chemical industry, food and other industries. [0003] Glycyrrhiza licorice...

Claims

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Application Information

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IPC IPC(8): C07J63/00
CPCC07J63/008
Inventor 朱荣耀华梦云
Owner 无锡市本草机械科技有限公司
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