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Application of urine-derived stem cells in preparation of biological preparation and medicine for treating non-alcoholic fatty liver disease, losing weight and lowering lipid

A non-alcoholic, biological preparation technology, applied in the field of prevention and treatment of drugs, to achieve the effect of inhibiting lipid deposition and glucose tolerance

Pending Publication Date: 2021-11-02
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of urine-derived stem cells (USCs) in the treatment of NAFLD has not been reported yet.

Method used

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  • Application of urine-derived stem cells in preparation of biological preparation and medicine for treating non-alcoholic fatty liver disease, losing weight and lowering lipid
  • Application of urine-derived stem cells in preparation of biological preparation and medicine for treating non-alcoholic fatty liver disease, losing weight and lowering lipid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: USCs reduce the increase in liver weight caused by high fat.

[0018] 1.1 Experimental method

Embodiment approach 1

[0019] Embodiment 1: USCs inhibit high-fat diet-induced hepatic lipid deposition

[0020] 1.1 Experimental method:

[0021] 1.1.1 Isolation and culture of USCs

[0022] In order to study the effects of USCs in various diseases, the present invention has successfully established a complete set of methods for USCs isolation, culture, subculture, and identification, and has isolated and cultured more than 60 cases of human-derived urinary stem cells.

[0023] (1) Urine collection: clean the urethral opening, and collect 200 mL of clean midstream urine (non-morning urine). (Definition of mid-segment urine: mid-segment urine refers to clinical urine culture. When urine is collected, the urethra is washed clean by the first urine, and the middle urine is taken as a sample for culture. The purpose is to prevent urine from being polluted. Here, The special medium for USCs was prepared as follows: serum-free keratinocyte medium (K-SFM) and precursor cell medium (EFM) were mixed accor...

Embodiment 2

[0036] Example 2: USCs can reduce glucose tolerance and insulin resistance in HFD mice

[0037] 2.1 Experimental method:

[0038] 2.1.1 Glucose tolerance test (GTT) changes in mice detected by blood glucose meter

[0039] After tail vein injection of USCs and HFD feeding for 8w, fasting for 16h. At 0, 15, 30, 60 and 120 minutes after the intraperitoneal injection of glucose solution (2 g / kg body weight), blood was taken from the tail of the blood glucose meter to measure the blood glucose concentration.

[0040] 2.1.2 Blood glucose meter detects changes in mice (insulin tolerance test, ITT)

[0041] After tail vein injection of USCs and HFD feeding for 8w, fasting for 16h. Insulin solution (0.75U / kg body weight) was injected intraperitoneally, and at 0, 15, 30, 60 and 120 minutes after that, blood was taken from the tail of the blood glucose meter to measure the blood glucose concentration.

[0042] 2.2 Experimental results:

[0043] Experiments in mice showed that USCs c...

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Abstract

The invention relates to the technical field of application of prevention and treatment medicines, in particular to an application of urine-derived stem cells in preparation of a biological agent and a medicine for treating non-alcoholic fatty liver disease, losing weight and lowering lipid. The urine-derived stem cells are used for preparing non-alcoholic fatty liver treatment injections, infusion solutions or other pharmaceutical preparations containing the urine-derived stem cells. Experimental results show that the urine-derived stem cells (USCs) can significantly inhibit formation of high fat-induced NAFLD. It is found that USCs can significantly inhibit lipid deposition in the liver of an HFD mouse; the USCs can significantly relieve glucose tolerance and insulin resistance of HFD mice. NAFLD is a problem which increasingly affects human health; particularly, NAFLD is a common abnormal liver function troubling the health of the elderly; in addition, the USCs are obtained from a patient, so that side effects caused by allogenic stem cell treatment can be effectively avoided, and the USCs have a relatively good prospect when being used for treating the NAFLD.

Description

technical field [0001] The invention relates to the technical field of application of prevention and treatment drugs, in particular to the application of urine-derived stem cells in the preparation of biological preparations and drugs for non-alcoholic fatty liver treatment, weight loss and lipid-lowering. Background technique [0002] Epidemiology points out that non-alcoholic fatty liver disease (NAFLD) is a type of metabolic syndrome caused by excessive lipid deposition in the liver. NAFLD occurs frequently in people in China, Europe, North America and Australia. NAFLD is closely related to obesity, hyperlipidemia, type 2 diabetes, heart disease and other abnormal glucose and lipid metabolism diseases, and diseases of the elderly, and NAFLD can even develop into liver cancer. Therefore, NAFLD is an important metabolic disease that endangers human health. [0003] However, there is currently no specific clinical drug against NAFLD. Therefore, the research and developmen...

Claims

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Application Information

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IPC IPC(8): A61K35/22A61P1/16A61P3/04A61P3/06
CPCA61K35/22A61P1/16A61P3/04A61P3/06
Inventor 陈添兴
Owner NANTONG UNIVERSITY
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