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Method for tracing origin of Largehead Atractylodes Rh based on nano-effect visual sensor

A technology of origin traceability and nano-effects, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions. and other problems, to achieve the effect of small size, fast response and high cost

Active Publication Date: 2021-10-26
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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Problems solved by technology

[0003] At present, there are traditional analysis methods such as high performance liquid chromatography, gas chromatography, and high performance capillary electrophoresis to detect the origin of Atractylodes macrocephala at home and abroad. These analysis methods have high accuracy and reliability, but they need expensive equipment and Strict laboratory conditions, sample processing is not only complicated, but also the analysis speed is very slow, which cannot meet the needs of rapid detection in the market

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  • Method for tracing origin of Largehead Atractylodes Rh based on nano-effect visual sensor
  • Method for tracing origin of Largehead Atractylodes Rh based on nano-effect visual sensor
  • Method for tracing origin of Largehead Atractylodes Rh based on nano-effect visual sensor

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Embodiment 1

[0030] A method for tracing the origin of Atractylodes macrocephala based on a nano-effect visual sensor, the schematic diagram of which is shown in figure 1 , including the following steps:

[0031] 1) Preparation of nano-gold: 1mL concentration of 25mM HAuCl 4 The solution was mixed with 99mL of deionized water, and at a temperature of 110°C, quickly added 10mL with a concentration of 1.455×10 -2 M (0.0427g) of trisodium citrate solution; The resulting reaction system was refluxed for 30min under vigorous stirring, and the reaction solution turned red and stirred to room temperature; Centrifuged at a speed of 3000rpm for 10min to remove larger nanoparticles, and Centrifuge at a rate of 7000rpm for 10min to remove unreacted chemical substances in the solution for purification, and the obtained AuNPs are redissolved in ultrapure water to obtain a gold nano solution with a concentration of 7nmol / L;

[0032] 2) Take 13 samples of Atractylodes Rhizoma from three producing areas...

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Abstract

The method comprises the following steps: sequentially introducing nanogold and Cr < 3 + > into extracting solutions of Largehead Atractylodes Rh samples from different producing areas, and according to the difference of aggregation degrees of the nanogold in an obtained reaction system and the difference of colors of the reaction system caused by the difference of the aggregation degrees of the nanogold in the reaction system, constructing the relationship between the production place of the Largehead Atractylodes Rh sample and the color change information, so as to realize visual identification of the production place of the Largehead Atractylodes Rh. The sensor constructed by the invention has the advantages of small size, high precision, quick response, visualization and the like, can quickly realize accurate discrimination of the production place of Largehead Atractylodes Rh, can effectively solve the problems of high cost, complex operation, long detection time and the like in the existing detection method, and is suitable for popularization and application.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis and detection, and in particular relates to a method for tracing the origin of Atractylodes macrocephala based on a nano-effect visual sensor. Background technique [0002] Atractylodes Rhizoma Atractylodes Rhizome is the dried rhizome of Atractylodes Rhizoma Atractylodes, mainly produced in Zhejiang, Hunan, Hubei, Anhui and other places. Among them, Pan'an, Xinchang and Shengzhou in Zhejiang have a long history of planting Atractylodes Rhizoma Atractylodes Rhizome, and are considered to be the authentic production areas of Atractylodes Rhizoma Atractylodes. The quality of Atractylodes macrocephala produced in Yuqian (now Hangzhou, Lin'an, Zhejiang) is the best, and it is specially called "Yushu" and is listed as a single species. Due to the large number of production areas of Atractylodes macrocephala in my country, the quality of medicinal materials is uneven, and the habitats and clim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 付海燕刘瑞杨小龙杨健陈亨业王游游
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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