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Salt-tolerant heterotrophic nitrification and aerobic denitrification bacterium and application thereof

A technology of aerobic denitrification bacteria and aerobic denitrification, applied in the field of environmental microorganisms, can solve the problem of high construction cost

Active Publication Date: 2021-10-26
江西中江环保集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, different processes such as traditional biological denitrification, nitrification and denitrification are required to be carried out in two reactors, the construction cost is high, and each step needs to add carbon sources, adjust acid and alkali, etc.

Method used

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  • Salt-tolerant heterotrophic nitrification and aerobic denitrification bacterium and application thereof
  • Salt-tolerant heterotrophic nitrification and aerobic denitrification bacterium and application thereof
  • Salt-tolerant heterotrophic nitrification and aerobic denitrification bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Screening, Identification and Preservation of Klebsiella oxytoca YZ-12

[0026] (1) Screening of Klebsiella oxytoca YZ-12

[0027] Take water samples from the aerobic pool of the sewage treatment station of the farm, add 10% inoculum amount to the sterile primary screening medium, culture at 30°C, 120rpm shaker at constant temperature for 24 hours, and pass three times continuously. Dilute the culture solution of 3 consecutive cultures with sterile water for 10 -5 、10 -6 、10 -7 times, spread it on the primary screening solid medium, and incubate at a constant temperature of 30°C for 1 to 2 days. A single colony grows, and the single colony is repeatedly picked and streaked again, and finally streaked on the slant of the LB solid medium test tube, and stored in a refrigerator at 4°C.

[0028] The formula of described initial screening culture medium is as follows: C 4 h 4 Na 2 o 4 ·6H 2 O 8.5g / L, KNO 3 1g / L, MgSO 4 ·7H 2 O1g / L, KH 2 PO 4 1g / L, CaCl 2 2H 2...

Embodiment 2

[0036] Nitrogen removal effect of Klebsiella oxytoca YZ-12 under different conditions

[0037] (1) Detection of the influence of carbon source on the denitrification effect of Klebsiella oxytoca YZ-12

[0038] Seed solution preparation: take the Klebsiella oxytoca YZ-12 stored in the refrigerator at 4°C in Example 1, inoculate it in a 250mL conical flask containing 50mL of seed medium, and inoculate it at 28-35°C, 120-200r / min Under certain conditions, cultivate for 12-16 hours to obtain the seed solution.

[0039] Nitrogen removal effect detection: use glucose, sucrose, sodium acetate, methanol, sodium succinate as the only carbon source to prepare ammonia nitrogen medium and nitrate nitrogen medium, NH 4 + -N concentration is 350mg / L, NO 3 - -N concentration is 415mg / L, add seed liquid according to 5% inoculum amount, cultivate for 72h at 28-35°C, 120-200r / min, take samples every 24h and centrifuge at 8000rpm, take supernatant and use Nessler's reagent to spectrophotomet...

Embodiment 3

[0054] Effect of Klebsiella oxytoca YZ-12 on denitrification of aquaculture wastewater

[0055] Use sucrose to adjust the C / N of the aquaculture wastewater to be 10, add the seed solution of Example 2 according to the inoculum amount of 5%, cultivate it for 72 hours at 28~35°C and 120~200r / min, take a sample and centrifuge at 8000rpm every 24h, and take the supernatant Determination of NH with Nessler's reagent spectrophotometry 4 + -N concentration, NO detected by ion chromatography 3 - -N concentration. Results Aquaculture wastewater NH 4 + -N removal rate is 95.15%, NO 3 - -N removal rate is 99.76%.

[0056] The water quality of the culture wastewater is as follows: pH value 7.8, COD value 2031mg / L, ammonia nitrogen 362mg / L, nitrate nitrogen 23.8mg / L, nitrite nitrogen 0.167mg / L.

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Abstract

The invention provides a salt-tolerant heterotrophic nitrification and aerobic denitrification bacterium, which belongs to the technical field of biology, and has a preservation number of GDMCC NO.61505. According to the salt-tolerant heterotrophic nitrification and aerobic denitrification bacterium, Klebsiella oxytoca YZ-12 is separated from culture wastewater, the strain has salt resistance, heterotrophic nitrification and aerobic denitrification capabilities, and can still grow and partially denitrify when the salinity is 30 g / L, and the denitrification rate for ammonia nitrogen wastewater with the concentration of 150 mg / L or below and nitrate nitrogen wastewater with the concentration of 500 mg / L or below is 97% or above. The strain provided by the invention has a potential application prospect in sewage treatment.

Description

technical field [0001] The invention relates to a salt-tolerant heterotrophic nitrifying aerobic denitrifying bacteria and its application, belonging to the technical field of environmental microorganisms. Background technique [0002] With the eutrophication of water body becoming more and more prominent, the prevention and control of nitrogen pollution has been paid more and more attention by people. At present, the sewage denitrification treatment methods mainly include chemical method, biochemical method and biophysical-physicochemical combined process, among which biological denitrification has become the main means of sewage denitrification because of its economical, efficient and non-residual pollution characteristics. However, different processes such as traditional biological denitrification, nitrification and denitrification are required to be carried out in two reactors, the construction cost is high, and each step needs to add carbon sources, adjust acid and alka...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/22
CPCC02F3/34C02F3/02Y02W10/10
Inventor 雷强张燕孙燕封志飞刘啟明
Owner 江西中江环保集团股份有限公司
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