Nano-liter liquid phase system with automatic cleaning function and control method

Abstract

The invention discloses a nano-liter liquid phase system with an automatic cleaning function and a control method. The nano-liter liquid phase system comprises a nano-liter liquid phase, a switching valve assembly, a chromatographic column assembly and an elution device; the switching valve assembly comprises a first switching valve and a second switching valve; the chromatographic column assembly comprises a first chromatographic column and a second chromatographic column which are arranged in parallel; two ends of the first chromatographic column and the second chromatographic column are respectively connected with the first switching valve and the second switching valve; the elution device is connected with the first switching valve and the second switching valve; and a nano-liter liquid phase is configured to load samples to the first chromatographic column or the second chromatographic column through the first switching valve, and control the first switching valve and the second switching valve to switch between different positions according to the state information of the first chromatographic column and the second chromatographic column. Through the nano-liter liquid phase system, cleaning, sample loading and elution can be realized in a seamless manner, the invalid waiting time of a mass spectrum can be avoided, 24-hour uninterrupted data acquisition of the mass spectrum is realized, and the utilization rate of a mass spectrum platform is improved.

Description

technical field [0001] The invention belongs to the field of biochemical process equipment, in particular to a nanoliter liquid phase system with automatic cleaning function and a control method thereof. Background technique [0002] At present, nanoliter liquid chromatography high-resolution mass spectrometry detection is the main detection method of proteomics. Among them, the method of nanoliter liquid chromatography can be summarized into three stages: preparation, analysis, and cleaning in chronological order. The mass spectrometer can only collect data during the analysis phase, and the preparation and cleaning phases are in a standby state. [0003] The main disadvantage of the existing equipment is that the sum of the preparation and cleaning time is almost equivalent to the analysis time. During the preparation and cleaning time, because the mass spectrometer has not obtained the corresponding detection data, no useful information is produced during the preparation ...

AI Technical Summary

Problems solved by technology

[0003] The main disadvantage of the existing equipment is that the sum of the preparation and cleaning time is almost equivalent to the analysis time. During the preparation and cleaning time, because the mass spectrometer has not obtained the corresponding detection data, no useful information is produced during the preparation and cleaning process. data

It can be seen that in the prior art, mass spectrometers are waiting for a long time to obtain test data, resulting in a large amount of invalid waiting time, which makes the work efficiency of nanoliter liquid chromatography high-resolution mass spectrometry detection low

Images