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Serum creatinine detection reagent ball and serum creatinine detection chip

A detection reagent and detection chip technology, applied in measurement devices, color/spectral property measurement, material analysis by observing the influence on chemical indicators, etc. Anti-interference ability, high precision effect

Pending Publication Date: 2021-10-19
GENRUI BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the process of realizing the present invention, the inventors found that in the prior art, the measurement results of blood creatinine content are easily interfered by other substances in the blood. Such substances are due to their strong reducing properties or the structure of the chromogen substrate in the reaction with Trinder Similarly, hydrogen peroxide will be consumed during the Trinder reaction, which will affect the measurement results

Method used

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  • Serum creatinine detection reagent ball and serum creatinine detection chip
  • Serum creatinine detection reagent ball and serum creatinine detection chip
  • Serum creatinine detection reagent ball and serum creatinine detection chip

Examples

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preparation example Construction

[0052] In some embodiments, the preparation method of the reagent ball (the second reagent ball or the third reagent ball) comprises the following steps:

[0053] S11. Drop the droplet of the first reagent (the second reagent or the third reagent) in liquid nitrogen, so that the droplet of the first reagent (the second reagent or the third reagent) forms an ice ball;

[0054] For example, the droplet of the first reagent (the second reagent or the third reagent) can be dropped in liquid nitrogen through a glue dispenser, so that the droplet condenses into an ice ball in the liquid nitrogen. Those skilled in the art can adjust the size of the droplet dropped into the liquid nitrogen according to actual needs, and adjust the volume of the ice puck by controlling the size of the droplet. Optionally, in some embodiments, the volume of the ice ball is 2.5-3.5 μl, for example, it may be 2.5 μl, 3 μl or 3.5 μl.

[0055] S12. Freeze-drying the ice balls to obtain freeze-dried reagent...

Embodiment 1

[0067] The raw materials of the blood creatinine detection reagent ball in this embodiment include the following components:

[0068] Each component of the first reagent is as follows: the HEPES damping fluid of 20mmol / L, the sodium chloride of 10g / L, the 2,4,6-tribromo-3-hydroxybenzoic acid of 40g / L, the Tween of 10g / L -20, 30g / L fructose, 500KU / L creatinase, 60KU / L sarcosine oxidase, 100KU / L ascorbate oxidase and 150g / L trehalose;

[0069] The components of the second reagent are as follows: 100mmol / L HEPES buffer, 5g / L sodium chloride, 0.2g / L 4-aminoantipyridine, 1g / L potassium ferrocyanide, 0.5g The Tween-20 of / L, the bovine serum albumin of 50g / L, the peroxidase of 50KU / L and the trehalose of 200g / L;

[0070] The third reagent: 1kg / L of the second reagent and 200KU / L of creatinase.

[0071] In this embodiment, the above-mentioned preparation method of blood creatinine detection reagent balls is used to prepare freeze-dried reagent balls, and the volumes of the first re...

Embodiment 2

[0074] The difference between this embodiment and embodiment 1 is as follows:

[0075] The components of the first reagent are as follows: 100mmol / L phosphate buffer, 6g / L sodium chloride, 30g / L 2,4,6-tribromo-3-hydroxybenzoic acid, 0.1g / L Triton X-100, 40g / L glycerin, 100KU / L creatinase, 200KU / L sarcosine oxidase, 50KU / L ascorbate oxidase and 200g / L water-soluble starch;

[0076] The components of the second reagent are as follows: 20mmol / L 3-morpholine propanesulfonic acid buffer, 10g / L sodium chloride, 10g / L 4-aminoantipyridine, 0.005g / L Red pigment disruptor, 10g / L Triton X-100, 10g / L glycerin, 200KU / L peroxidase and 100g / L water-soluble starch;

[0077] The third reagent: 1kg / L of the second reagent and 500KU / L of creatinase.

[0078] The volumes of the first reagent ball, the second reagent ball and the third reagent ball are about 2.5ul.

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Abstract

The invention relates to the technical field of serum creatinine determination, and in particular relates to a serum creatinine detection reagent ball and a serum creatinine detection chip. According to the serum creatinine detection reagent ball and the serum creatinine detection chip containing the serum creatinine detection reagent ball, interference of vitamin C in a detection sample can be eliminated through ascorbic acid oxidase, and interference of bilirubin can be eliminated by taking a bilirubin interference removing agent as an oxidizing agent; interference of endogenous creatine, sarcosine and peroxide existing in a detection sample is eliminated through creatinase, sarcosine oxidase and peroxidase respectively. The serum creatinine detection reagent ball and the serum creatinine detection chip provided by the invention have relatively strong anti-interference capability on original interferents in serum and drug interferents taken by patients, and the precision of a detection result is high.

Description

technical field [0001] The invention relates to the technical field of serum creatinine measurement, in particular to a blood creatinine detection reagent ball and a blood creatinine detection chip. Background technique [0002] The sources of serum creatinine (Cr, creatinine) include exogenous creatinine ingested from food and endogenous creatinine produced by muscle metabolism in the body. Creatinine is slowly formed primarily from creatine through irreversible non-enzymatic dehydration and released into the blood. Creatinine is a small molecular substance that can be filtered by the glomeruli and rarely absorbed in the renal tubules. The creatinine produced in the body every day is almost all excreted with urine, and is generally not affected by the amount of urine. The content of serum creatinine is closely related to the total amount of muscle in the body and is not easily affected by diet. [0003] Clinically, detecting the content of serum creatinine is one of the ...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/01G01N21/31
CPCG01N21/78G01N21/01G01N21/3103
Inventor 周慧欣汪晨宇牟健陈明
Owner GENRUI BIOTECH INC
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