KASP molecular marker closely linked with major QTL of pod wall tenderness of peas and development method and application of KASP molecular marker

Abstract

The invention provides a KASP molecular marker closely linked with a major QTL of pod wall tenderness of peas. The KASP molecular marker comprises a first KASP molecular marker and / or a second KASP molecular marker, wherein the first KASP molecular marker is used for specifically detecting a 297000666 site of a Chr1LG6 chromosome of the peas, and the second KASP molecular marker is used for specifically detecting a 302006525 site of the Chr1LG6 chromosome of the peas. The invention further provides a related development method and application. The KASP molecular marker closely linked with the major QTL of the pod wall tenderness of the peas, provided by the invention, is closely linked with the major QTL of the pod wall tenderness of the peas, so that the pod wall tenderness can be quickly and accurately pre-judged at an early growth stage of the peas, molecular marker-assisted selection with a soft pod type or a hard pod type as a breeding target is realized, the accuracy of breeding selection is improved, the breeding process is accelerated, and thus, the KASP molecular marker has an important application value in quality improvement on edible pea pods and is applicable to large-scale popularization and application.

Description

technical field [0001] The present invention relates to the technical field of bioinformatics and molecular marker genetics, more specifically, to the field of pea KASP molecular marker technology, in particular to a KASP molecular marker closely linked with the main effect QTL of pea pod wall tenderness and its development method and apply. Background technique [0002] Pea (Pisum sativum L.) belongs to the genus Pea of ​​the family Fabaceae. It is one of the four most important soybean crops in the world and the second largest edible bean in my country. Its planting area covers almost all parts of the country. According to the type of pod wall and edible parts, peas are divided into grain peas and pod-eating peas. According to the statistics of the Food and Agriculture Organization of the United Nations, the total annual output of peas in my country reaches 14.5 million tons, accounting for more than 40% of the world's total output (FAOSTAT 2020). [0003] The pod is the...

AI Technical Summary

Problems solved by technology

[0006]However, limited by the complexity of pea omics research and pod wall formation, molecular markers related to it have not been reported so far. Variety improvement using molecular marker-assisted selection still not possible

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