Angelica sinensis fermented liquid with antioxidative, antiaging and regulating intestinal flora functions, preparation method and application thereof
A technology for fermentation liquid and angelica, applied in the field of angelica fermentation liquid and its preparation, can solve the problems of slow treatment process, long decoction time, bitter taste and the like, and achieve the effects of scavenging free radicals, increasing content and relieving aging
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[0038] The invention provides a preparation method of Angelica sinensis fermentation liquid, comprising the following steps:
[0039] (1) after the Angelica sinensis is soaked in water, decoct, filter and collect the filtrate, add water to the filter residue, continue to decoct, filter and collect the filtrate, merge the filtrate twice, concentrate, centrifuge, and obtain the angelica water extract;
[0040] (2) Fermented Angelica water extract:
[0041] Lactobacillus plantarum LZU-J-TSL6 and LZU-S-ZCJ were mixed and inoculated into the water extract of Angelica sinensis for fermentation to obtain Angelica sinensis fermentation broth.
[0042] In the step (1) of the present invention, the soaking time is preferably 8 to 16 hours, more preferably 10 to 14 hours, and even more preferably 12 hours.
[0043] In the step (1) of the present invention, the volume ratio of the first decoction of Angelica sinensis and water is preferably 1:8 to 12, more preferably 1:8 to 10, even more...
Embodiment 1
[0052] Add 10 times the volume of water to Angelica sinensis, soak for 12 hours, decoct for 1 hour, filter and collect the filtrate, add 8 times the volume of water to the filter residue, continue to decoct for 1 hour, filter and collect the filtrate, combine the two filtrates, and concentrate the filtrate to 0.6g / mL juice at 4°C , 3000rmp centrifugation, take the supernatant, set aside. Lactobacillus plantarum LZU-J-TSL6 and LZU-S-ZCJ were mixed at a volume ratio of 1:3, and the concentration of the two strains was 5 × 10 12 CFU / mL, inoculated in the above-mentioned Angelica sinensis water extract of 1 mL of extract solution prepared per 0.6 g of Angelica sinensis at an inoculation amount of 4%. Under the condition of fermentation temperature of 36 ℃, fermented for 72 hours to obtain Angelica sinensis fermentation broth.
Embodiment 2
[0054] Angelica sinensis was added to 8 times the volume of water, soaked for 8 hours, decocted for 0.5 hours, filtered to collect the filtrate, the filter residue was added to 6 times the volume of water, continued to be decocted for 0.5 hours, the filtrate was collected by filtration, the two filtrates were combined, and the filtrate was concentrated to 0.4g / mL juice, Centrifuge at 3000rmp at 4°C, take the supernatant and set aside. Lactobacillus plantarum LZU-J-TSL6 and LZU-S-ZCJ were mixed in a volume ratio of 3:1, and the concentration of the two strains was 1 × 10 12 CFU / mL, inoculated in the above-mentioned Angelica sinensis water extract prepared by 1 mL of extract solution per 0.4 g of Angelica sinensis at an inoculation amount of 3%. Under the condition of fermentation temperature of 37℃, fermented for 100h to obtain Angelica sinensis fermentation broth.
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