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Angelica sinensis fermented liquid with antioxidative, antiaging and regulating intestinal flora functions, preparation method and application thereof

A technology for fermentation liquid and angelica, applied in the field of angelica fermentation liquid and its preparation, can solve the problems of slow treatment process, long decoction time, bitter taste and the like, and achieve the effects of scavenging free radicals, increasing content and relieving aging

Active Publication Date: 2022-07-22
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the field of traditional Chinese medicine, it has a good curative effect on the treatment of chronic diseases, but it has been criticized for its slow treatment process, bitter taste, and long cooking time.
Moreover, most of the active ingredients of traditional Chinese medicine are wrapped in dense cell walls, which are difficult to dissolve

Method used

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  • Angelica sinensis fermented liquid with antioxidative, antiaging and regulating intestinal flora functions, preparation method and application thereof
  • Angelica sinensis fermented liquid with antioxidative, antiaging and regulating intestinal flora functions, preparation method and application thereof
  • Angelica sinensis fermented liquid with antioxidative, antiaging and regulating intestinal flora functions, preparation method and application thereof

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preparation example Construction

[0038] The invention provides a preparation method of Angelica sinensis fermentation liquid, comprising the following steps:

[0039] (1) after the Angelica sinensis is soaked in water, decoct, filter and collect the filtrate, add water to the filter residue, continue to decoct, filter and collect the filtrate, merge the filtrate twice, concentrate, centrifuge, and obtain the angelica water extract;

[0040] (2) Fermented Angelica water extract:

[0041] Lactobacillus plantarum LZU-J-TSL6 and LZU-S-ZCJ were mixed and inoculated into the water extract of Angelica sinensis for fermentation to obtain Angelica sinensis fermentation broth.

[0042] In the step (1) of the present invention, the soaking time is preferably 8 to 16 hours, more preferably 10 to 14 hours, and even more preferably 12 hours.

[0043] In the step (1) of the present invention, the volume ratio of the first decoction of Angelica sinensis and water is preferably 1:8 to 12, more preferably 1:8 to 10, even more...

Embodiment 1

[0052] Add 10 times the volume of water to Angelica sinensis, soak for 12 hours, decoct for 1 hour, filter and collect the filtrate, add 8 times the volume of water to the filter residue, continue to decoct for 1 hour, filter and collect the filtrate, combine the two filtrates, and concentrate the filtrate to 0.6g / mL juice at 4°C , 3000rmp centrifugation, take the supernatant, set aside. Lactobacillus plantarum LZU-J-TSL6 and LZU-S-ZCJ were mixed at a volume ratio of 1:3, and the concentration of the two strains was 5 × 10 12 CFU / mL, inoculated in the above-mentioned Angelica sinensis water extract of 1 mL of extract solution prepared per 0.6 g of Angelica sinensis at an inoculation amount of 4%. Under the condition of fermentation temperature of 36 ℃, fermented for 72 hours to obtain Angelica sinensis fermentation broth.

Embodiment 2

[0054] Angelica sinensis was added to 8 times the volume of water, soaked for 8 hours, decocted for 0.5 hours, filtered to collect the filtrate, the filter residue was added to 6 times the volume of water, continued to be decocted for 0.5 hours, the filtrate was collected by filtration, the two filtrates were combined, and the filtrate was concentrated to 0.4g / mL juice, Centrifuge at 3000rmp at 4°C, take the supernatant and set aside. Lactobacillus plantarum LZU-J-TSL6 and LZU-S-ZCJ were mixed in a volume ratio of 3:1, and the concentration of the two strains was 1 × 10 12 CFU / mL, inoculated in the above-mentioned Angelica sinensis water extract prepared by 1 mL of extract solution per 0.4 g of Angelica sinensis at an inoculation amount of 3%. Under the condition of fermentation temperature of 37℃, fermented for 100h to obtain Angelica sinensis fermentation broth.

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Abstract

The invention belongs to the technical field of traditional Chinese medicine fermentation, and provides an angelica fermented liquid with anti-oxidation, anti-aging and intestinal flora regulation functions, and a preparation method and application thereof. Filtrate, filter residue, add water, continue to decoct, filter and collect the filtrate, combine the filtrates twice, concentrate, centrifuge to obtain Angelica sinensis water extract; Ferment Angelica sinensis water extract: mix LZU-J-TSL6 and LZU-S-ZCJ with Lactobacillus plantarum , inoculated in the water extract of Angelica sinensis and fermented to obtain Angelica sinensis fermentation broth. The angelica fermented liquid obtained by the preparation method of the present invention has anti-oxidative and anti-aging effects, better scavenging effects of free radicals, can regulate intestinal flora, and has good health care effects.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine fermentation, in particular to an angelica fermented liquid with anti-oxidation, anti-aging and intestinal flora regulation functions, and a preparation method and application thereof. Background technique [0002] Aerobic organisms in the world are gradually exposed to the environment produced by oxidants or electrophiles, which are affected by the process of enzymatic reactions in the body and external harmful substances. These oxidative poisons can have serious effects on the body. In order to avoid these bad effects, the body has evolved a complex but stable antioxidant defense system to maintain the redox reaction in the body. The excessive production of ROS (reactive oxygen species) by cells in the body and ROS produced by electrophiles will induce free radical chain reactions, cross-linking and polymerizing biological macromolecules in cells, and generate malondialdehyd...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/04A61K36/232A61P39/06A61P1/00C12R1/25
CPCC12P1/04A61K36/232A61P39/06A61P1/00A61K2236/331A61K2236/39A61K2236/51A61K2236/53A61K2236/19
Inventor 张春江卢雪蕊李君翔
Owner LANZHOU UNIVERSITY
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